dc.contributor.author
Borowski, Sophia
dc.contributor.author
Tirado-Gonzalez, Irene
dc.contributor.author
Freitag, Nancy
dc.contributor.author
Garcia, Mariana G.
dc.contributor.author
Barrientos, Gabriela
dc.contributor.author
Blois, Sandra M.
dc.date.accessioned
2021-02-01T15:21:44Z
dc.date.available
2021-02-01T15:21:44Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/29446
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-29192
dc.description.abstract
Immune cells [e. g., dendritic cells (DC) and natural killer (NK) cells] are critical players during the pre-placentation stage for successful mammalian pregnancy. Proper placental and fetal development relies on balanced DC-NK cell interactions regulating immune cell homing, maternal vascular expansion, and trophoblast functions. Previously, we showed thatin vivodisruption of the uterine NK cell-DC balance interferes with the decidualization process, with subsequent impact on placental and fetal development leading to fetal growth restriction. Glycans are essential determinants of reproductive health and the glycocode expressed in a particular compartment (e.g., placenta) is highly dependent on the cell type and its developmental and pathological state. Here, we aimed to investigate the maternal and placental glycovariation during the pre- and post-placentation period associated with disruption of the NK cell-DC dynamics during early pregnancy. We observed that depletion of NK cells was associated with significant increases of O- and N-linked glycosylation and sialylation in the decidual vascular zone during the pre-placental period, followed by downregulation of core 1 and poly-LacNAc extended O-glycans and increased expression of branched N-glycans affecting mainly the placental giant cells and spongiotrophoblasts of the junctional zone. On the other hand, expansion of DC induced a milder increase of Tn antigen (truncated form of mucin-type O-glycans) and branched N-glycan expression in the vascular zone, with only modest changes in the glycosylation pattern during the post-placentation period. In both groups, this spatiotemporal variation in the glycosylation pattern of the implantation site was accompanied by corresponding changes in galectin-1 expression. Our results show that pre- and post- placentation implantation sites have a differential glycopattern upon disruption of the NK cell-DC dynamics, suggesting that immune imbalance early in gestation impacts placentation and fetal development by directly influencing the placental glycocode.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
dendritic cells
en
dc.subject
natural killer cells
en
dc.subject
implantation
en
dc.subject
glycoimmunology
en
dc.subject
placentation
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Altered Glycosylation Contributes to Placental Dysfunction Upon Early Disruption of the NK Cell-DC Dynamics
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
1316
dcterms.bibliographicCitation.doi
10.3389/fimmu.2020.01316
dcterms.bibliographicCitation.journaltitle
Frontiers in Immunology
dcterms.bibliographicCitation.originalpublishername
Frontiers Media SA
dcterms.bibliographicCitation.volume
11
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
32760395
dcterms.isPartOf.eissn
1664-3224