dc.contributor.author
Stoll, Anna
dc.contributor.author
Iannone, Michele
dc.contributor.author
De Gregorio, Giuseppina
dc.contributor.author
Molaioni, Francesco
dc.contributor.author
Torre, Xavier de la
dc.contributor.author
Botrè, Francesco
dc.contributor.author
Parr, Maria Kristina
dc.date.accessioned
2021-01-28T17:57:51Z
dc.date.available
2021-01-28T17:57:51Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/29394
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-29140
dc.description.abstract
Anabolic androgenic steroids (AAS) are prohibited as doping substances in sports by the World Anti-Doping Agency. Concentrations and concentration ratios of endogenous AAS (steroid profile markers) in urine samples collected from athletes are used to detect their administration. Certain (non-prohibited) drugs have been shown to influence the steroid profile and thereby sophisticate anti-doping analysis. It was shown in vitro that the non-steroidal anti-inflammatory drug (NSAID) indomethacin inhibits selected steroid-biotransformations catalyzed by the aldo-keto reductase (AKR) 1C3, which plays a key role in the endogenous steroid metabolism. Kinetic parameters for the indomethacin-mediated inhibition of the AKR1C3 catalyzed reduction in etiocholanolone were determined in vitro using two comparing methods. As NSAIDs are very frequently used (not only) by athletes, the inhibitory impact of indomethacin intake on the steroid metabolism was evaluated, and steroid profile alterations were detected in vivo (one male and one female volunteer). Significant differences between samples collected before, during or after the intake of indomethacin for selected steroid profile markers were observed. The presented results are of relevance for the interpretation of results from doping control analysis. Additionally, the administration of NSAIDs should be carefully reconsidered due to their potential as endocrine disruptors.
en
dc.format.extent
19 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
aldo-keto reductases
en
dc.subject
endocrine disruption
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::540 Chemie::540 Chemie und zugeordnete Wissenschaften
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::615 Pharmakologie, Therapeutik
dc.title
Influence of Indomethacin on Steroid Metabolism: Endocrine Disruption and Confounding Effects in Urinary Steroid Profiling of Anti-Doping Analyses
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
463
dcterms.bibliographicCitation.doi
10.3390/metabo10110463
dcterms.bibliographicCitation.journaltitle
Metabolites
dcterms.bibliographicCitation.number
11
dcterms.bibliographicCitation.originalpublishername
MDPI
dcterms.bibliographicCitation.volume
10
dcterms.bibliographicCitation.url
https://doi.org/10.3390/metabo10110463
refubium.affiliation
Biologie, Chemie, Pharmazie
refubium.affiliation.other
Institut für Pharmazie

refubium.note.author
Die Publikation wurde aus Open Access Publikationsgeldern der Freien Universität Berlin gefördert.
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
2218-1989