dc.contributor.author
Mwamuye, Micky M.
dc.contributor.author
Odongo, David
dc.contributor.author
Kazungu, Yvette
dc.contributor.author
Kindoro, Fatuma
dc.contributor.author
Gwakisa, Paul
dc.contributor.author
Bishop, Richard P.
dc.contributor.author
Nijhof, Ard M.
dc.contributor.author
Obara, Isaiah
dc.date.accessioned
2020-11-16T13:17:40Z
dc.date.available
2020-11-16T13:17:40Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/28880
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-28629
dc.description.abstract
Buffalo-derived Theileria parva can ‘break through’ the immunity induced by the infection and treatment vaccination method (ITM) in cattle. However, no such ‘breakthroughs’ have been reported in northern Tanzania where there has been long and widespread ITM use in pastoralist cattle, and the Cape buffalo (Syncerus caffer) is also present. We studied the exposure of vaccinated and unvaccinated cattle in northern Tanzania to buffalo-derived T. parva using p67 gene polymorphisms and compared this to its distribution in vaccinated cattle exposed to buffalo-derived T. parva in central Kenya, where vaccine ‘breakthroughs’ have been reported. Additionally, we analysed the CD8+ T cell target antigen Tp2 for positive selection. Our results showed that 10% of the p67 sequences from Tanzanian cattle (n = 39) had a buffalo type p67 (allele 4), an allele that is rare among East African isolates studied so far. The percentage of buffalo-derived p67 alleles observed in Kenyan cattle comprised 19% of the parasites (n = 36), with two different p67 alleles (2 and 3) of presumptive buffalo origin. The Tp2 protein was generally conserved with only three Tp2 variants from Tanzania (n = 33) and five from Kenya (n = 40). Two Tanzanian Tp2 variants and two Kenyan Tp2 variants were identical to variants present in the trivalent Muguga vaccine. Tp2 evolutionary analysis did not show evidence for positive selection within previously mapped epitope coding sites. The p67 data indicates that some ITM-vaccinated cattle are protected against disease induced by a buffalo-derived T. parva challenge in northern Tanzania and suggests that the parasite genotype may represent one factor explaining this.
en
dc.format.extent
12 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Theileria parva
en
dc.subject
Antigen diversity
en
dc.subject
Cape Buffalo
en
dc.subject
Live vaccine
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche
dc.title
Variant analysis of the sporozoite surface antigen gene reveals that asymptomatic cattle from wildlife-livestock interface areas in northern Tanzania harbour buffalo-derived T. parva
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.doi
10.1007/s00436-020-06902-1
dcterms.bibliographicCitation.journaltitle
Parasitology Research
dcterms.bibliographicCitation.number
11
dcterms.bibliographicCitation.pagestart
3817
dcterms.bibliographicCitation.pageend
3828
dcterms.bibliographicCitation.volume
119
dcterms.bibliographicCitation.url
https://doi.org/10.1007/s00436-020-06902-1
refubium.affiliation
Veterinärmedizin
refubium.affiliation.other
Institut für Parasitologie und Tropenveterinärmedizin
refubium.funding
Springer Nature DEAL
refubium.note.author
Die Publikation wurde aus Open Access Publikationsgeldern der Freien Universität Berlin gefördert.
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.issn
0932-0113
dcterms.isPartOf.eissn
1432-1955
refubium.resourceType.provider
WoS-Alert