dc.contributor.author
Heimesaat, Markus M.
dc.contributor.author
Genger, Claudia
dc.contributor.author
Biesemeier, Nina
dc.contributor.author
Klove, Sigri
dc.contributor.author
Weschka, Dennis
dc.contributor.author
Mousavi, Soraya
dc.contributor.author
Bereswill, Stefan
dc.date.accessioned
2020-09-11T12:12:11Z
dc.date.available
2020-09-11T12:12:11Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/28087
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-27837
dc.description.abstract
Human infections with the food-borne enteropathogens Campylobacter are progressively rising. Recent evidence revealed that pre-existing intestinal inflammation facilitates enteropathogenic infection subsequently exacerbating the underlying disease. Given that only little is known about C. coli-host interactions and particularly during intestinal inflammation, the aim of the present study was to survey gastrointestinal colonization properties, gut microbiota changes and pro-inflammatory sequelae upon peroral C. coli-infection of IL-10-/- mice with chronic colitis. C. coli colonized the gastrointestinal tract of mice with varying efficiencies until day 28 post-infection and induced macroscopic and microscopic inflammatory changes as indicated by shorter colonic lengths, more distinct histopathological changes in the colonic mucosa and higher numbers of apoptotic colonic epithelial cells when compared to mock-infected controls. Furthermore, not only colonic innate and adaptive immune cell responses, but also enhanced systemic TNF-α secretion could be observed following C. coli as opposed to mock challenge. Notably, C. coli induced intestinal inflammatory sequelae were accompanied with gut microbiota shifts towards higher commensal enterobacterial loads in the infected gut lumen. Moreover, the pathogen translocated from the intestinal tract to extra-intestinal tissue sites in some cases, but never to systemic compartments. Hence, C. coli accelerates inflammatory immune responses in IL-10-/- mice with chronic colitis.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Campylobacter coli
en
dc.subject
murine chronic colitis
en
dc.subject
host-pathogen interaction
en
dc.subject
aged IL-10-/- mice
en
dc.subject
intestinal immunopathology
en
dc.subject
bacterial colonization
en
dc.subject
gut microbiota changes
en
dc.subject
enterobacterial overgrowth
en
dc.subject
intestinal and systemic immune responses
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Inflammatory Immune Responses and Gut Microbiota Changes Following Campylobacter coli Infection of IL-10 -/- Mice with Chronic Colitis
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
560
dcterms.bibliographicCitation.doi
10.3390/pathogens9070560
dcterms.bibliographicCitation.journaltitle
Pathogens
dcterms.bibliographicCitation.originalpublishername
MDPI AG
dcterms.bibliographicCitation.volume
9
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
32664563
dcterms.isPartOf.eissn
2076-0817