dc.contributor.author
Lettau, Marie
dc.contributor.author
Wiedemann, Annika
dc.contributor.author
Schrezenmeier, Eva Vanessa
dc.contributor.author
Giesecke-Thiel, Claudia
dc.contributor.author
Dörner, Thomas
dc.date.accessioned
2020-07-06T09:22:15Z
dc.date.available
2020-07-06T09:22:15Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/27428
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-27184
dc.description.abstract
Background: Memory B cell (mBC) induction and maintenance is one of the keys to long-term protective humoral immunity. MBCs are fundamental to successful medical interventions such as vaccinations and therapy in autoimmunity. However, their lifestyle and anatomic residence remain enigmatic in humans. Extrapolation from animal studies serves as a conceptual basis but might be misleading due to major anatomical distinctions between species.
Methods and findings: Multicolor immunofluorescence stainings on fixed and unfixed frozen tissue sections were established using primary antibodies coupled to haptens and secondary signal amplification. The simultaneous detection of five different fluorescence signals enabled the localization and characterization of human CD27+CD20+Ki67- mBCs for the first time within one section using laser scanning microscopy. As a result, human tonsillar mBCs were initially identified within their complex microenvironment and their relative location to naïve B cells, plasma cells and T cells could be directly studied and compared to the human splenic mBC niche. In all investigated tonsils (n = 15), mBCs appeared to be not only located in a so far subepithelial defined area but were also follicle associated with a previous undescribed gradual decline towards the follicular mantle comparable to human spleen. However, mBC areas around secondary follicles with large germinal centers (GCs) in tonsils showed interruptions and a general widening towards the epithelium while in spleen the mBC-containing marginal zones (MZ) around smaller GCs were relatively broad and symmetrical. Considerably fewer IgM+IgD+/- pre-switch compared to IgA+ or IgG+ post-switch mBCs were detected in tonsils in contrast to spleen.
Conclusions: This study extends existing insights into the anatomic residence of human mBCs showing structural similarities of the superficial follicular area in human spleen and tonsil. Our data support the debate of renaming the human splenic MZ to 'superficial zone' in order to be aware of the differences in rodents and, moreover, to consider this term equally for the human palatine tonsil.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
B-Lymphocytes
en
dc.subject
Cellular Microenvironment
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Human CD27+ Memory B Cells Colonize a Superficial Follicular Zone in the Palatine Tonsils With Similarities to the Spleen. A Multicolor Immunofluorescence Study of Lymphoid Tissue
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
e0229778
dcterms.bibliographicCitation.doi
10.1371/journal.pone.0229778
dcterms.bibliographicCitation.journaltitle
PLoS ONE
dcterms.bibliographicCitation.number
3
dcterms.bibliographicCitation.originalpublishername
Public Library of Science (PLoS)
dcterms.bibliographicCitation.volume
15
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
32187186
dcterms.isPartOf.eissn
1932-6203