dc.contributor.author
Chen, Shuai
dc.contributor.author
Einspanier, Ralf
dc.contributor.author
Schoen, Jennifer
dc.date.accessioned
2020-03-03T14:16:40Z
dc.date.available
2020-03-03T14:16:40Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/26826
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-26584
dc.description.abstract
Recently, we established a protocol for the cultivation of primary porcine oviduct epithelial cells (POEC), which promoted tissue-like morphology for a prolonged culture period. The present study focuses on developing this model into a comprehensive, standardized culture system, as a candidate tool for reproductive toxicity testing and basic research. We cultivated POEC isolated from 25 animals in our culture system for both 3 and 6 weeks and systematically analyzed effects of medium conditioning, supplementation with standardized sera, and culture duration in both freshly isolated and cryopreserved cells. The differentiation status was evaluated via histomorphometry, transepithelial electrical resistance (TEER) measurement, and expression analyses. The culture system possessed high reproducibility, more than 95% of cultures achieved a fully differentiated phenotype. Cells recapitulated in vivo–like morphology and ultrastructure from 3 to 6 weeks. Cryopreservation of the cells prior to cultivation did not affect culture quality of POEC. Employment of conditioned medium ensured optimal promotion of POEC differentiation, and different standardized sera induced fully differentiated phenotypes. Consistent TEER establishment indicated the presence and maintenance of cell type–specific intercellular junctions. The functionality of POEC was proven by consistent mucin secretion and stable expression of selected markers over the whole culture duration. We conclude that POEC are suitable for experiments from 3 weeks up to at least 6 weeks of culture. Therefore, this culture system could be used for in vitro estrous cycle simulation and long-term investigation of toxic effects on oviduct epithelium.
en
dc.format.extent
8 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject
oviduct epithelium
en
dc.subject
standardized culture system
en
dc.subject
long-term culture
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::530 Physik::530 Physik
dc.title
Long-term culture of primary porcine oviduct epithelial cells: Validation of a comprehensive in vitro model for reproductive science
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.doi
10.1016/j.theriogenology.2013.07.011
dcterms.bibliographicCitation.journaltitle
Theriogenology
dcterms.bibliographicCitation.number
8
dcterms.bibliographicCitation.pagestart
862
dcterms.bibliographicCitation.pageend
869
dcterms.bibliographicCitation.volume
80
dcterms.bibliographicCitation.url
https://doi.org/10.1016/j.theriogenology.2013.07.011
refubium.affiliation
Veterinärmedizin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.issn
0093-691X
dcterms.isPartOf.eissn
1879-3231