dc.contributor.author
Greco, Gabriele
dc.contributor.author
Amasheh, Salah
dc.contributor.author
Shen, Zanming
dc.contributor.author
Lu, Zhongyan
dc.contributor.author
Aschenbach, Jörg R.
dc.date.accessioned
2019-09-27T12:40:48Z
dc.date.available
2019-09-27T12:40:48Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/25667
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-25431
dc.description.abstract
Epidermal growth factor (EGF) and glucagon‐like peptides (GLP) modulate the tight junctions (TJ) of the intestinal epithelial barrier (EB) of monogastric animals. This work tried to elucidate whether GLP‐1, GLP‐2 and EGF can affect the EB of the rumen. Ovine ruminal epithelia were incubated in Ussing chambers for 7 hr with 25 or 250 nM of either GLP‐1 or GLP‐2 on the serosal side, with 2.5 nM of EGF on the serosal side or with 0.25 or 2.5 nM EGF on the mucosal side. No treatment affected tissue conductance. Short‐circuit current (Isc) was affected by time and treatment and their interactions. Only 250 nM of either GLP‐1 or GLP‐2 decreased Isc in certain periods compared with 25 nM GLP‐1 or 0.25 nM mucosally applied EGF; however, not when compared to control epithelia. Fluorescein flux rates (Jfluor) of ruminal epithelia were affected by treatment, time and time × treatment interaction. The time × treatment interaction was based on an increase in Jfluor between the first and last hour in epithelia incubated with 25 nM GLP‐1 or GLP‐2 and in epithelia incubated with EGF. After 7 hr incubation, claudin‐7 mRNA expression was downregulated in all treatments. Claudin‐1 mRNA was upregulated after incubation with 2.5 nM EGF on the serosal side, claudin‐4 mRNA was downregulated by 2.5 nM EGF on the mucosal side, and occludin mRNA was increased after incubation with 250 nM GLP‐2. The protein abundance of all tested TJ proteins was not influenced by treatment. We conclude that GLP‐1, GLP‐2, and EGF have no obvious acute effects on the EB of ruminal epithelia under simulated physiological conditions ex vivo. However, by decreasing the mRNA expression of claudin‐7 and partly affecting other TJ proteins, they may modulate EB in the longer term or under certain conditions.
en
dc.format.extent
12 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
epidermal growth factor
en
dc.subject
epithelial barrier
en
dc.subject
glucagon‐like peptide
en
dc.subject
tight junction
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::590 Tiere (Zoologie)::599 Mammalia (Säugetiere)
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::616 Krankheiten
dc.title
Effects of glucagon‐like peptides 1 and 2 and epidermal growth factor on the epithelial barrier of the rumen of adult sheep
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.doi
10.1111/jpn.13200
dcterms.bibliographicCitation.journaltitle
Journal of Animal Physiology and Animal Nutrition
dcterms.bibliographicCitation.url
https://doi.org/10.1111/jpn.13200
refubium.affiliation
Veterinärmedizin
refubium.affiliation.other
Institut für Veterinär-Physiologie
refubium.funding
DEAL Wiley
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
1439-0396
refubium.resourceType.provider
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