dc.contributor.author
El-Duah, Philip
dc.contributor.author
Meyer, Benjamin
dc.contributor.author
Sylverken, Augustina
dc.contributor.author
Owusu, Michael
dc.contributor.author
Gottula, Lina Theresa
dc.contributor.author
Yeboah, Richmond
dc.contributor.author
Lamptey, Jones
dc.contributor.author
Frimpong, Yaw Oppong
dc.contributor.author
Burimuah, Vitus
dc.contributor.author
Folitse, Raphael
dc.contributor.author
Agbenyega, Olivia
dc.contributor.author
Oppong, Samuel
dc.contributor.author
Adu-Sarkodie, Yaw
dc.contributor.author
Drosten, Christian
dc.date.accessioned
2019-06-12T12:05:18Z
dc.date.available
2019-06-12T12:05:18Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/24753
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-2513
dc.description.abstract
Known human coronaviruses are believed to have originated in animals and made use of intermediate hosts for transmission to humans. The intermediate hosts of most of the human coronaviruses are known, but not for HCoV-NL63. This study aims to assess the possible role of some major domestic livestock species as intermediate hosts of HCoV-NL63. We developed a testing algorithm for high throughput screening of livestock sera with ELISA and confirmation with recombinant immunofluorescence assay testing for antibodies against HCoV-NL63 in livestock. Optimization of the ELISA showed a capability of the assay to significantly distinguish HCoV-NL63 from HCoV-229E (U = 27.50, p < 0.001) and HCoV-OC43 (U = 55.50, p < 0.001) in coronavirus-characterized sera. Evaluation of the assay with collected human samples showed no significant difference in mean optical density values of immunofluorescence-classified HCoV-NL63-positive and HCoV-NL63-negative samples (F (1, 215) = 0.437, p = 0.509). All the top 5% (n = 8) most reactive human samples tested by ELISA were HCoV-NL63 positive by immunofluorescence testing. In comparison, only a proportion (84%, n = 42) of the top 25% were positive by immunofluorescence testing, indicating an increased probability of the highly ELISA reactive samples testing positive by the immunofluorescence assay. None of the top 5% most ELISA reactive livestock samples were positive for HCoV-NL63-related viruses by immunofluorescence confirmation. Ghanaian domestic livestock are not likely intermediate hosts of HCoV-NL63-related coronaviruses.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
intermediate host
en
dc.subject
immunofluorescence
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Development of a Whole-Virus ELISA for Serological Evaluation of Domestic Livestock as Possible Hosts of Human Coronavirus NL63
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
43
dcterms.bibliographicCitation.doi
10.3390/v11010043
dcterms.bibliographicCitation.journaltitle
Viruses
dcterms.bibliographicCitation.number
1
dcterms.bibliographicCitation.originalpublishername
MDPI AG
dcterms.bibliographicCitation.volume
11
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
30634419
dcterms.isPartOf.issn
1999-4915