dc.contributor.author
Brockman, Tobias
dc.contributor.author
Blanchard, Véronique
dc.contributor.author
Heretsch, Philipp
dc.contributor.author
Brockmann, Claudia
dc.contributor.author
Bertelmann, Eckart
dc.date.accessioned
2019-04-16T10:27:37Z
dc.date.available
2019-04-16T10:27:37Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/24432
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-2204
dc.description.abstract
Purpose To investigate the photochemical degradation of trypan blue (TB) and to identify decomposition products. Methods Defined solution samples of TB and a mixture with lutein/zeaxanthin were exposed to blue light. Thermal degradation processes were ruled out using controls not subjected to irradiation. All samples were analyzed using optical microscopy, UVNis spectroscopy, matrix assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry and nuclear magnetic resonance (NMR) spectrometry. Degradation kinetics were determined based on changes in absorbance; intermediates were identified by analyzing mass differences of characteristic fragment ion peaks within the fragmentation patterns, and assignments were verified by NMR. Results TB demonstrated a photochemical degradation, which can be triggered by lutein/zeaxanthin. Intermediates vary depending on the presence of lutein/zeaxanthin. The self-sensitized photodegradation of TB occurs under generation of dimethyl sulfate and presumed formation of phenol. In contrast, within the presence of lutein/zeaxanthin the decomposition of TB indicates the formation of methoxyamine and sulfonyl arin. Thermal degradation processes were not observed. Conclusions TB demonstrated a photodegradation that may be triggered by lutein/zeaxanthin and results in the formation of cytotoxic decomposition products. Our findings contribute to understand degradation mechanisms of TB and may elucidate previous clinical and experimental observations of cellular toxicity after TB application.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
photochemical degradation
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Photochemical degradation of trypan blue
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
e0195849
dcterms.bibliographicCitation.doi
10.1371/journal.pone.0195849
dcterms.bibliographicCitation.journaltitle
PLoS ONE
dcterms.bibliographicCitation.number
4
dcterms.bibliographicCitation.originalpublishername
Public Library of Science (PLoS)
dcterms.bibliographicCitation.volume
13
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
29634764
dcterms.isPartOf.issn
1932-6203