dc.contributor.author
Matz, Mareen
dc.contributor.author
Heinrich, Frederik
dc.contributor.author
Lorkowski, Christine
dc.contributor.author
Wu, Kaiyin
dc.contributor.author
Klotsche, Jens
dc.contributor.author
Zhang, Qiang
dc.contributor.author
Lachmann, Nils
dc.contributor.author
Durek, Pawel
dc.contributor.author
Budde, Klemens
dc.contributor.author
Mashreghi, Mir-Farzin
dc.date.accessioned
2019-04-10T13:20:13Z
dc.date.available
2019-04-10T13:20:13Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/24361
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-2133
dc.description.abstract
Interstitial fibrosis/tubular atrophy (IFTA) is associated with reduced allograft survival, whereas antibody-mediated rejection (ABMR) is the major cause for renal allograft failure. To identify specific microRNAs and their regulation involved in these processes, total RNA from blood cells of 16 kidney transplanted (KTx) patients with ABMR, stable graft function (SGF) and with T-cell mediated rejection (TCMR) was isolated. MicroRNA expression was determined by high-throughput sequencing. Differentially expressed candidate microRNAs were analyzed with RT-PCR in patients with SGF (n = 53), urinary tract infection (UTI) (n = 17), borderline rejection (BL) (n = 19), TCMR (n = 40), ABMR (n = 22) and IFTA (n = 30). From the 301 detected microRNAs, 64 were significantly regulated between the three cohorts. Selected candidate microRNAs miR-223-3p, miR-424-3p and miR-145-5p distin-guished TCMR and ABMR from SGF, but not from other pathologies. Most importantly, miR-145-5p expression in IFTA patients was significantly downregulated and displayed a high diagnostic accuracy compared to SGF alone (AUC = 0.891) and compared to SGF, UTI, BL, TCMR and ABMR patients combined (AUC = 0.835), which was verified by cross-validation. The identification of miR-145-5p as IFTA specific marker in blood constitutes the basis for evaluating this potentially diagnostic microRNA as biomarker in studies including high numbers of patients and different pathologies and also the further analysis of fibrosis causing etiologies after kidney transplantation.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
MicroRNA regulation in blood cells of renal transplanted patients with interstitial fibrosis/tubular atrophy and antibody-mediated rejection
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
e0201925
dcterms.bibliographicCitation.doi
10.1371/journal.pone.0201925
dcterms.bibliographicCitation.journaltitle
PLoS ONE
dcterms.bibliographicCitation.number
8
dcterms.bibliographicCitation.originalpublishername
Public Library of Science (PLoS)
dcterms.bibliographicCitation.volume
13
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
30102719
dcterms.isPartOf.issn
1932-6203