dc.contributor.author
Wernecke, Laura
dc.contributor.author
Keckeis, Susanne
dc.contributor.author
Reichhart, Nadine
dc.contributor.author
Strauß, Olaf
dc.contributor.author
Salchow, Daniel J.
dc.date.accessioned
2019-04-01T09:41:25Z
dc.date.available
2019-04-01T09:41:25Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/24242
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-2014
dc.description.abstract
PURPOSE. Posterior capsule opacification (PCO) is a complication after cataract surgery, particularly in children. Epithelial-mesenchymal transition (EMT) of lens epithelial cells, mediated by transforming growth factor beta (TGF beta), contributes to PCO. However, its pathogenesis in children is poorly understood. We correlated cell growth in culture with patient characteristics, studied gene expression of pediatric lens epithelial cells (pLEC), and examined the effects of TGF beta-2 on these cells in vitro. METHODS. Clinical characteristics of children with cataracts correlated with growth behavior of pLEC in vitro. mRNA expression of epithelial (alpha B-crystallin, connexin-43) and mesenchymal (alpha(V)-integrin, alpha-smooth muscle actin, collagen-I alpha 2, fibronectin-1) markers was quantified in pLEC and in cell line HLE-B3 in the presence and absence of TGF beta-2. RESULTS. Fifty-four anterior lens capsules from 40 children aged 1 to 180 months were obtained. Cell outgrowth occurred in 44% of the capsules from patients <= 12 months and in 33% of capsules from children aged 13 to 60 months, but in only 6% of capsules from children over 60 months. TGF beta-2 significantly upregulated expression of alpha B-crystallin (HLE-B3), alpha(V)-integrin (HLE-B3), collagen-I alpha 2, and fibronectin-1 (in pLEC and HLE-B3 cells). CONCLUSIONS. Patient characteristics correlated with growth behavior of pLEC in vitro, paralleling a higher clinical incidence of PCO in younger children. Gene expression profiles of pLEC and HLE-B3 suggest that upregulation of alpha(V)-integrin, collagen-I alpha 2, and fibronectin-1 are involved in EMT.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
posterior capsule opacification
en
dc.subject
epithelial-mesenchymal transformation
en
dc.subject
lens epithelial cells
en
dc.subject
transforming growth factor beta-2
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Epithelial-mesenchymal transdifferentiation in pediatric lens epithelial cells
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.doi
10.1167/iovs.18-23789
dcterms.bibliographicCitation.journaltitle
Investigative ophthalmology & visual science
dcterms.bibliographicCitation.number
15
dcterms.bibliographicCitation.originalpublishername
ARVO
dcterms.bibliographicCitation.pagestart
5785
dcterms.bibliographicCitation.pageend
5794
dcterms.bibliographicCitation.volume
59
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
30521667
dcterms.isPartOf.issn
1552-5783