dc.contributor.author
Wight, Darren J.
dc.contributor.author
Wallaschek, Nina
dc.contributor.author
Sanyal, Anirban
dc.contributor.author
Weller, Sandra K.
dc.contributor.author
Flamand, Louis
dc.contributor.author
Kaufer, Benedikt B.
dc.date.accessioned
2019-01-07T11:23:26Z
dc.date.available
2019-01-07T11:23:26Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/23639
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-1425
dc.description.abstract
Human herpesvirus-6A and -6B (HHV-6A and -6B) are two closely related betaherpesviruses that infect humans. Upon primary infection they establish a life-long infection termed latency, where the virus genome is integrated into the telomeres of latently infected cells. Intriguingly, HHV-6A/B can integrate into germ cells, leading to individuals with inherited chromosomally-integrated HHV-6 (iciHHV-6), who have the HHV-6 genome in every cell. It is known that telomeric repeats flanking the virus genome are essential for integration; however, the protein factors mediating integration remain enigmatic. We have previously shown that the putative viral integrase U94 is not essential for telomere integration; thus, we set out to assess the contribution of potential viral recombination proteins U41 and U70 towards integration. We could show that U70 enhances dsDNA break repair via a homology-directed mechanism using a reporter cell line. We then engineered cells to produce shRNAs targeting both U41 and U70 to inhibit their expression during infection. Using these cells in our HHV-6A in vitro integration assay, we could show that U41/U70 were dispensable for telomere integration. Furthermore, additional inhibition of the cellular recombinase Rad51 suggested that it was also not essential, indicating that other cellular and/or viral factors must mediate telomere integration.
en
dc.format.extent
10 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
human herpesvirus 6A
en
dc.subject
telomere integration
en
dc.subject
recombination
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::579 Mikroorganismen, Pilze, Algen
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::616 Krankheiten
dc.title
Viral Proteins U41 and U70 of Human Herpesvirus 6A Are Dispensable for Telomere Integration
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
656
dcterms.bibliographicCitation.doi
10.3390/v10110656
dcterms.bibliographicCitation.journaltitle
Viruses
dcterms.bibliographicCitation.number
11
dcterms.bibliographicCitation.volume
10
refubium.affiliation
Veterinärmedizin
refubium.affiliation.other
Institut für Virologie
![No reasonable confidence value was returned from the authority](/cache_202e45ad85b55efaeb29160f63cd3f3b/themes/FuCD/images/authority_control/invisible.gif)
refubium.funding
Institutional Participation
refubium.funding.id
MDPI
refubium.note.author
Die Publikation wurde aus Open Access Publikationsgeldern der Freien Universität Berlin und der DFG gefördert.
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.issn
1999-4915