dc.contributor.author
Chen, Chao
dc.contributor.author
De Masi, Raffaella
dc.contributor.author
Lintermann, Ruth
dc.contributor.author
Wirthmueller, Lennart
dc.date.accessioned
2018-11-07T12:34:48Z
dc.date.available
2018-11-07T12:34:48Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/23181
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-973
dc.description.abstract
Proteins of the Poly(ADP-Ribose) Polymerase (PARP) family modify target proteins by covalent attachment of ADP-ribose moieties onto amino acid side chains. In Arabidopsis, PARP proteins contribute to repair of DNA lesions and modulate plant responses to various abiotic and biotic stressors. Arabidopsis PARP1 and PARP2 are nuclear proteins and given that their molecular weights exceed the diffusion limit of nuclear pore complexes, an active import mechanism into the nucleus is likely. Here we use confocal microscopy of fluorescent protein-tagged Arabidopsis PARP2 and PARP2 deletion constructs in combination with site-directed mutagenesis to identify a nuclear localization sequence in PARP2 that is required for nuclear import. We report that in co-immunoprecipitation assays PARP2 interacts with several isoforms of the importin-α group of nuclear transport adapters and that PARP2 binding to IMPORTIN-α2 is mediated by the identified nuclear localization sequence. Our results demonstrate that PARP2 is a cargo protein of the canonical importin-α/β nuclear import pathway.
dc.format.extent
11 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Poly(ADP-Ribose) Polymerase
en
dc.subject
nuclear localization sequence
en
dc.subject
nucleo-cytoplasmic transport
en
dc.subject
Arabidopsis thaliana
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::580 Pflanzen (Botanik)::583 Magnoliopsida (Zweikeimblättrige)
dc.title
Nuclear Import of Arabidopsis Poly(ADP-Ribose) Polymerase 2 Is Mediated by Importin-α and a Nuclear Localization Sequence Located Between the Predicted SAP Domains
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
1581
dcterms.bibliographicCitation.doi
10.3389/fpls.2018.01581
dcterms.bibliographicCitation.journaltitle
Frontiers in Plant Science
dcterms.bibliographicCitation.volume
9
dcterms.bibliographicCitation.url
https://doi.org/10.3389/fpls.2018.01581
refubium.affiliation
Biologie, Chemie, Pharmazie
refubium.affiliation.other
Institut für Biologie
de
refubium.funding
Institutional Participation
refubium.funding.id
Frontiers
refubium.note.author
Der Artikel wurde in einer reinen Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.issn
1664-462X