dc.contributor.author
Hobbs, Matthew
dc.contributor.author
King, Andrew
dc.contributor.author
Salinas, Ryan
dc.contributor.author
Chen, Zhiliang
dc.contributor.author
Tsangaras, Kyriakos
dc.contributor.author
Greenwood, Alex D.
dc.contributor.author
Johnson, Rebecca N.
dc.contributor.author
Belov, Katherine
dc.contributor.author
Wilkins, Marc R.
dc.contributor.author
Timms, Peter
dc.date.accessioned
2018-06-08T11:09:47Z
dc.date.available
2018-01-15T12:24:12.669Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/21733
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-25021
dc.description.abstract
The koala retrovirus (KoRV) is implicated in several diseases affecting the
koala (Phascolarctos cinereus). KoRV provirus can be present in the genome of
koalas as an endogenous retrovirus (present in all cells via germline
integration) or as exogenous retrovirus responsible for somatic integrations
of proviral KoRV (present in a limited number of cells). This ongoing invasion
of the koala germline by KoRV provides a powerful opportunity to assess the
viral strategies used by KoRV in an individual. Analysis of a high-quality
genome sequence of a single koala revealed 133 KoRV integration sites. Most
integrations contain full-length, endogenous provirus; KoRV-A subtype. The
second most frequent integrations contain an endogenous recombinant element
(recKoRV) in which most of the KoRV protein-coding region has been replaced
with an ancient, endogenous retroelement. A third set of integrations, with
very low sequence coverage, may represent somatic cell integrations of KoRV-A,
KoRV-B and two recently designated additional subgroups, KoRV-D and KoRV-E.
KoRV-D and KoRV-E are missing several genes required for viral processing,
suggesting they have been transmitted as defective viruses. Our results
represent the first comprehensive analyses of KoRV integration and variation
in a single animal and provide further insights into the process of
retroviral-host species interactions.
en
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Molecular evolution
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft
dc.title
Long-read genome sequence assembly provides insight into ongoing retroviral
invasion of the koala germline
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Scientific Reports. - 7 (2017), Artikel Nr. 15838
dcterms.bibliographicCitation.doi
10.1038/s41598-017-16171-1
dcterms.bibliographicCitation.url
http://www.nature.com/articles/s41598-017-16171-1
refubium.affiliation
Veterinärmedizin
de
refubium.mycore.fudocsId
FUDOCS_document_000000028803
refubium.note.author
Der Artikel wurde in einer reinen Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000009333
dcterms.accessRights.openaire
open access