dc.contributor.author
Anderson, Josephine L. C.
dc.contributor.author
Gautier, Thomas
dc.contributor.author
Nijstad, Niels
dc.contributor.author
Toelle, Markus
dc.contributor.author
Schuchardt, Mirjam
dc.contributor.author
van der Giet, Markus
dc.contributor.author
Tietge, Uwe J. F.
dc.date.accessioned
2018-06-08T11:07:22Z
dc.date.available
2017-03-21T13:02:14.470Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/21656
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-24944
dc.description.abstract
Atherosclerotic cardiovascular disease (CVD) represents the largest cause of
mortality in end-stage renal disease (ESRD). CVD in ESRD is not explained by
classical CVD risk factors such as HDL cholesterol mass levels making
functional alterations of lipoproteins conceivable. HDL functions in
atheroprotection by promoting reverse cholesterol transport (RCT), comprising
cholesterol efflux from macrophage foam cells, uptake into hepatocytes and
final excretion into the feces. ESRD-HDL (n = 15) were compared to healthy
control HDL (n = 15) for their capacity to promote in vitro (i) cholesterol
efflux from THP-1 macrophage foam cells and (ii) SR-BI-mediated selective
uptake into ldla[SR-BI] cells as well as (iii) in vivo RCT. Compared with HDL
from controls, ESRD-HDL displayed a significant reduction in mediating
cholesterol efflux (p < 0.001) and SR-BI-mediated selective uptake (p < 0.01),
two key steps in RCT. Consistently, also the in vivo capacity of ESRD-HDL to
promote RCT when infused into wild-type mice was significantly impaired (p <
0.01). In vitro oxidation of HDL from healthy controls with hypochloric acid
was able to fully mimic the impaired biological activities of ESRD-HDL. In
conclusion, we demonstrate that HDL from ESRD patients is dysfunctional in key
steps as well as overall RCT, likely due to oxidative modification.
en
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
End-stage renal disease
dc.subject
Preclinical research
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit
dc.title
High density lipoprotein (HDL) particles from end-stage renal disease patients
are defective in promoting reverse cholesterol transport
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Scientific Reports. - 7 (2017), Artikel Nr. 41481
dcterms.bibliographicCitation.doi
10.1038/srep41481
dcterms.bibliographicCitation.url
http://www.nature.com/articles/srep41481
refubium.affiliation
Charité - Universitätsmedizin Berlin
de
refubium.mycore.fudocsId
FUDOCS_document_000000026689
refubium.note.author
Der Artikel wurde in einer reinen Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000007936
dcterms.accessRights.openaire
open access