dc.contributor.author
Meyer, S.
dc.contributor.author
Schroeter, M. -A.
dc.contributor.author
Hahn, M. B.
dc.contributor.author
Solomun, T.
dc.contributor.author
Sturm, H.
dc.contributor.author
Kunte, H. J.
dc.date.accessioned
2018-06-08T11:01:19Z
dc.date.available
2017-09-18T11:03:10.357Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/21480
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-24772
dc.description.abstract
Strand breaks and conformational changes of DNA have consequences for the
physiological role of DNA. The natural protecting molecule ectoine is
beneficial to entire bacterial cells and biomolecules such as proteins by
mitigating detrimental effects of environmental stresses. It was postulated
that ectoine-like molecules bind to negatively charged spheres that mimic DNA
surfaces. We investigated the effect of ectoine on DNA and whether ectoine is
able to protect DNA from damages caused by ultraviolet radiation (UV-A). In
order to determine different isoforms of DNA, agarose gel electrophoresis and
atomic force microscopy experiments were carried out with plasmid pUC19 DNA.
Our quantitative results revealed that a prolonged incubation of DNA with
ectoine leads to an increase in transitions from supercoiled (undamaged) to
open circular (single-strand break) conformation at pH 6.6. The effect is pH
dependent and no significant changes were observed at physiological pH of 7.5.
After UV-A irradiation in ectoine solution, changes in DNA conformation were
even more pronounced and this effect was pH dependent. We hypothesize that
ectoine is attracted to the negatively charge surface of DNA at lower pH and
therefore fails to act as a stabilizing agent for DNA in our in vitro
experiments.
en
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Atomic force microscopy
dc.subject
Single-strand DNA breaks
dc.subject.ddc
500 Naturwissenschaften und Mathematik
dc.title
Ectoine can enhance structural changes in DNA in vitro
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Scientific Reports. - 7 (2017), Artikel Nr. 7170
dcterms.bibliographicCitation.doi
10.1038/s41598-017-07441-z
dcterms.bibliographicCitation.url
http://www.nature.com/articles/s41598-017-07441-z
refubium.affiliation
Physik
de
refubium.mycore.fudocsId
FUDOCS_document_000000027981
refubium.note.author
Der Artikel wurde in einer reinen Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000008756
dcterms.accessRights.openaire
open access