dc.contributor.author
Vierock, Johannes
dc.contributor.author
Grimm, Christiane
dc.contributor.author
Nitzan, Noam
dc.contributor.author
Hegemann, Peter
dc.date.accessioned
2018-06-08T10:32:04Z
dc.date.available
2017-10-10T12:55:15.866Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/20600
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-23901
dc.description.abstract
Channelrhodopsins are light-gated ion channels of green algae used for the
precise temporal and spatial control of transmembrane ion fluxes. The
channelrhodopsin Chrimson from Chlamydomonas noctigama allows unprecedented
deep tissue penetration due to peak absorption at 590 nm. We demonstrate by
electrophysiological recordings and imaging techniques that Chrimson is highly
proton selective causing intracellular acidification in HEK cells that is
responsible for slow photocurrent decline during prolonged illumination. We
localized molecular determinants of both high proton selectivity and red light
activation to the extracellular pore. Whereas exchange of Glu143 only drops
proton conductance and generates an operational Na-channel with 590 nm
activation, exchange of Glu139 in addition increased the open state lifetime
and shifted the absorption hypsochromic by 70 nm. In conjunction with Glu300
in the center and Glu124 and Glu125 at the intracellular end of the pore,
Glu139 contributes to a delocalized activation gate and stabilizes by long-
range interaction counterion configuration involving protonation of Glu165
that we identified as a key determinant of the large opsin shift in Chrimson.
en
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Ligand-gated ion channels
dc.subject
Membrane proteins
dc.subject
Molecular neuroscience
dc.subject
Permeation and transport
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit
dc.title
Molecular determinants of proton selectivity and gating in the red-light
activated channelrhodopsin Chrimson
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Scientific Reports. - 7 (2017), Artikel Nr. 9928
dcterms.bibliographicCitation.doi
10.1038/s41598-017-09600-8
dcterms.bibliographicCitation.url
http://www.nature.com/articles/s41598-017-09600-8
refubium.affiliation
Charité - Universitätsmedizin Berlin
de
refubium.mycore.fudocsId
FUDOCS_document_000000028239
refubium.note.author
Der Artikel wurde in einer reinen Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000008901
dcterms.accessRights.openaire
open access