dc.contributor.author
Roschanski, Nicole
dc.contributor.author
Fischer, Jennie
dc.contributor.author
Falgenhauer, Linda
dc.contributor.author
Pietsch, Michael
dc.contributor.author
Guenther, Sebastian
dc.contributor.author
Kreienbrock, Lothar
dc.contributor.author
Chakraborty, Trinad
dc.contributor.author
Pfeifer, Yvonne
dc.contributor.author
Guerra, Beatriz
dc.contributor.author
Roesler, Uwe H.
dc.date.accessioned
2018-06-08T10:27:37Z
dc.date.available
2018-04-13T11:46:23.460Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/20482
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-23785
dc.description.abstract
Carbapenems are last-resort antibiotics used in human medicine. The increased
detection of carbapenem-resistant Enterobacteriaceae (CRE) is therefore
worrying. In 2011 we reported the first livestock-associated VIM-1-producing
Salmonella (S.) enterica serovar Infantis (R3) isolate from dust, sampled in a
German chicken fattening farm. Due to this observation we retrospectively
investigated more than 536 stored bacterial cultures, isolated from 45 chicken
fattening farms during the years 2011 and 2012. After a non-selective
overnight incubation, the bacteria were transferred to selective media.
Escherichia (E.) coli and Salmonella growing on these media were further
investigated, including antibiotic susceptibility testing, carbapenemase gene
screening and whole genome sequencing (WGS). In total, four CRE were found in
three out of 45 investigated farms: Besides R3, one additional Salmonella
(G-336-1a) as well as two E. coli isolates (G-336-2, G-268-2). All but G-268-2
harbored the blaVIM-1 gene. Salmonella isolates R3 and G-336-1 were closely
related although derived from two different farms. All three blaVIM-1-encoding
isolates possessed identical plasmids and the blaVIM-1- containing transposon
showed mobility at least in vitro. In isolate G-268-2, the AmpC beta-lactamase
gene blaCMY-2 but no known carbapenemase gene was identified. However, a
transfer of the phenotypic resistance was possible. Furthermore, G-268-2
contained the mcr-1 gene, combining phenotypical carbapenem- as well as
colistin resistance in one isolate. Carbapenem-resistant Enterobacteriaceae
have been found in three out of 45 investigated chicken flocks. This finding
is alarming and emphasizes the importance of intervention strategies to
contain the environmental spread of resistant bacteria in animals and humans.
en
dc.format.extent
10 Seiten
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
antimicrobial resistance
dc.subject
VIM-1 producing Enterobacteriaceae
dc.subject.ddc
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::579 Mikroorganismen, Pilze, Algen
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::615 Pharmakologie, Therapeutik
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::636 Viehwirtschaft
dc.title
Retrospective Analysis of Bacterial Cultures Sampled in German Chicken-
Fattening Farms During the Years 2011–2012 Revealed Additional VIM-1
Carbapenemase-Producing Escherichia coli and a Serologically Rough Salmonella
enterica Serovar Infantis
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Frontiers in Microbiology 9 (2018), Art. 538
dcterms.bibliographicCitation.doi
10.3389/fmicb.2018.00538
dcterms.bibliographicCitation.url
http://dx.doi.org/10.3389/fmicb.2018.00538
refubium.affiliation
Veterinärmedizin
de
refubium.affiliation.other
Institut für Tier- und Umwelthygiene
refubium.funding
Institutional Participation
refubium.funding.id
Frontiers
refubium.mycore.fudocsId
FUDOCS_document_000000029574
refubium.note.author
Der Artikel wurde in einer reinen Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000009628
dcterms.accessRights.openaire
open access
dcterms.isPartOf.issn
1664-302X