dc.contributor.author
Folkert, J.
dc.contributor.author
Meresta, A.
dc.contributor.author
Gaber, T.
dc.contributor.author
Miksch, K.
dc.contributor.author
Buttgereit, F.
dc.contributor.author
Detert, J.
dc.contributor.author
Pischon, N.
dc.contributor.author
Gurzawska, K.
dc.date.accessioned
2018-06-08T10:21:55Z
dc.date.available
2017-02-16T12:00:27.737Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/20301
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-23605
dc.description.abstract
Abstract: A new strategy to improve osseointegration of implants is to
stimulate adhesion of bone cells, bone matrix formation, and mineralization at
the implant surface by modifying surface coating on the nanoscale level.
Plant-derived pectins have been proposed as potential candidates for surface
nanocoating of orthopedic and dental titanium implants due to 1) their
osteogenic stimulation of osteoblasts to mineralize and 2) their ability to
control pectin structural changes. The aim of this study was to evaluate in
vitro the impact of the nanoscale plant-derived pectin Rhamnogalacturonan-I
(RG-I) from potato on the osteogenic response of murine osteoblasts. RG-I from
potato pulps was isolated, structurally modified, or left unmodified. Tissue
culture plates were either coated with modified RG-I or unmodified RG-I or –
as a control – left uncoated. The effect of nanocoating on mice osteoblast-
like cells MC3T3-E1 and primary murine osteoblast with regard to
proliferation, osteogenic response in terms of mineralization, and gene
expression of Runt-related transcription factor 2 (Runx2), alkaline phosphate
(Alpl), osteocalcin (Bglap), α-1 type I collagen (Col1a1), and receptor
activator of NF-κB ligand (Rankl) were analyzed after 3, 7, 14, and 21 days,
respectively. Nanocoating with pectin RG-Is increased proliferation and
mineralization of MC3T3-E1 and primary osteoblast as compared to osteoblasts
cultured without nanocoating. Moreover, osteogenic transcriptional response of
osteoblasts was induced by nanocoating in terms of gene induction of Runx2,
Alpl, Bglap, and Col1a1 in a time-dependent manner – of note – to the highest
extent under the PA-coating condition. In contrast, Rankl expression was
initially reduced by nanocoating in MC3T3-E1 or remained unaltered in primary
osteoblast as compared to the uncoated controls. Our results showed that
nanocoating of implants with modified RG-I beneficially 1) supports
osteogenesis, 2) has the capacity to improve osseointegration of implants, and
is therefore 3) a potential candidate for nanocoating of bone implants.
en
dc.rights.uri
http://creativecommons.org/licenses/by-nc/3.0/
dc.subject
osseointegration
dc.subject
mineralization
dc.subject
Rhamnogalacturonan-I
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit
dc.title
Nanocoating with plant-derived pectins activates osteoblast response in vitro
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
International Journal of Nanomedicine. - 12 (2017), S. 239-249
dcterms.bibliographicCitation.doi
10.2147/IJN.S99020
dcterms.bibliographicCitation.url
http://doi.org/10.2147/IJN.S99020
refubium.affiliation
Charité - Universitätsmedizin Berlin
de
refubium.mycore.fudocsId
FUDOCS_document_000000026357
refubium.note.author
Der Artikel wurde in einer reinen Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000007690
dcterms.accessRights.openaire
open access