dc.contributor.author
Rykalina, Vera N.
dc.contributor.author
Shadrin, Alexey A.
dc.contributor.author
Amstislavskiy, Vyacheslav S.
dc.contributor.author
Rogaev, Evgeny I.
dc.contributor.author
Lehrach, Hans
dc.contributor.author
Borodina, Tatiana A.
dc.date.accessioned
2018-06-08T04:20:12Z
dc.date.available
2014-10-06T17:47:39.549Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/17079
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-21259
dc.description.abstract
Hybridization-based target enrichment protocols require relatively large
starting amounts of genomic DNA, which is not always available. Here, we
tested three approaches to pre-capture library preparation starting from 10 ng
of genomic DNA: (i and ii) whole-genome amplification of DNA samples with
REPLI-g (Qiagen) and GenomePlex (Sigma) kits followed by standard library
preparation, and (iii) library construction with a low input oriented ThruPLEX
kit (Rubicon Genomics). Exome capture with Agilent SureSelectXT2 Human AllExon
v4+UTRs capture probes, and HiSeq2000 sequencing were performed for test
libraries along with the control library prepared from 1 µg of starting DNA.
Tested protocols were characterized in terms of mapping efficiency, enrichment
ratio, coverage of the target region, and reliability of SNP genotyping.
REPLI-g- and ThruPLEX-FD-based protocols seem to be adequate solutions for
exome sequencing of low input samples
de
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject.ddc
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::576 Genetik und Evolution
dc.title
Exome Sequencing from Nanogram Amounts of Starting DNA
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
PLoS ONE. - 9 (2014), 7, Artikel Nr. e101154
dc.title.subtitle
Comparing Three Approaches
dcterms.bibliographicCitation.doi
10.1371/journal.pone.0101154
dcterms.bibliographicCitation.url
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0101154
refubium.affiliation
Charité - Universitätsmedizin Berlin
de
refubium.mycore.fudocsId
FUDOCS_document_000000021106
refubium.note.author
Der Artikel wurde in einer Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000004016
dcterms.accessRights.openaire
open access