dc.contributor.author
Khajavi, Noushafarin
dc.contributor.author
Reinach, Peter S.
dc.contributor.author
Skrzypski, Marek
dc.contributor.author
Lude, Anja
dc.contributor.author
Mergler, Stefan
dc.date.accessioned
2018-06-08T04:19:10Z
dc.date.available
2014-12-01T14:25:54.481Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/17044
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-21224
dc.description.abstract
Background/Aims: Ocular surface health depends on conjunctival epithelial
(HCjE) layer integrity since it protects against pathogenic infiltration and
contributes to tissue hydration maintenance. As the same increases in tear
film hyperosmolarity described in dry eye disease can increase corneal
epithelial transient receptor potential vanilloid type-1 (TRPV1) channel
activity, we evaluated its involvement in mediating an osmoprotective effect
by L-carnitine against such stress. Methods: Using siRNA gene silencing,
Ca2+imaging, planar patch- clamping and relative cell volume measurements, we
determined if the protective effects of this osmolyte stem from its
interaction with TRPV1. Results: TRPV1 activation by capsaicin (CAP) and an
increase in osmolarity to≈450 mOsM both induced increases in Ca2+levels. In
contrast, blocking TRPV1 activation with capsazepine (CPZ) fully reversed this
response. Similarly, L-carnitine (1 mM) also reduced underlying whole-cell
currents. In calcein-AM loaded cells, hypertonic-induced relative cell volume
shrinkage was fully blocked during exposure to L-carnitine. On the other hand,
in TRPV1 gene-silenced cells, this protective effect by L-carnitine was
obviated. Conclusion: The described L-carnitine osmoprotective effect is
elicited through suppression of hypertonic-induced TRPV1 activation leading to
increases in L-carnitine uptake through a described Na+-dependent L-carnitine
transporter.
en
dc.rights.uri
http://creativecommons.org/licenses/by-nc/3.0/de/
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit
dc.title
L-Carnitine Reduces in Human Conjunctival Epithelial Cells Hypertonic- Induced
Shrinkage through Interacting with TRPV1 Channels
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Cellular Physiology and Biochemistry. - 34 (2014), S. 790-803
dcterms.bibliographicCitation.url
http://www.karger.com/Article/Pdf/363043
refubium.affiliation
Charité - Universitätsmedizin Berlin
de
refubium.mycore.fudocsId
FUDOCS_document_000000021388
refubium.note.author
Der Artikel wurde in einer Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000004217
dcterms.accessRights.openaire
open access