Aufbauend auf dem professionsorientierten Kompetenzmodell für die zukunftsfähige Lehre nach Möller 2023 dient dieses Reflexionsportfolio der Auseinandersetzung und Reflexion von Lehramtsstudierenden und Lehrkräften mit einzelnen Facetten ihrer Profession und den notwendigen Kompetenzen, mit einem ausdifferenzierten Schwerpunkt auf aktuelle gesellschaftliche Transformationen und ihre Herausforderungen für Bildungsprozesse (in Form von Future Skills).

Channel belts form through the mobilization and deposition of sediments during the lateral migration of rivers. Channel-belt width and its temporal evolution are important for the hydraulics, hydrology, and ecology of landscapes, as well as for human activities such as farming, protecting infrastructure, and natural hazard mitigation. Yet, we currently lack a comprehensive theoretical description of the width evolution of channel belts. Here, we explore the predictions of a physics-based model of channel-belt width for the transient evolution of channel belts. The model applies to laterally unconfined channel belts in foreland areas as well as to laterally confined channel belts in mountain settings (here, channel-belt width equals valley floor width). The model builds on the assumption that the switching of direction of a laterally migrating channel can be described by a Poisson process, with a constant rate parameter related to channel hydraulics. As such, the lateral migration of the channel can be viewed as a nonstandard one-dimensional random walk. In other words, at each river cross section the river randomly moves either to the left or right at a given time. The model predicts three phases in the growth of channel belts. First, before the channel switches direction for the first time, the channel belt grows linearly. Second, as long as the current width is smaller than the steady-state width, growth follows an exponential curve on average. Finally, there is a drift phase, in which the channel-belt width grows with the square root of time. We exploit the properties of random walks to obtain equations for the distance from a channel that is unlikely to be inundated in a given time interval (law of the iterated logarithm), distributions of times the channel requires to return to its origin and to first arrive at a given position away from the origin, and the mean lateral drift speed of steady-state channel belts. All of the equations can be directly framed in terms of the channel's hydraulic properties, in particular its lateral transport capacity that quantifies the amount of material that the river can move in lateral migration per unit time and channel length. The distribution of sediment age within the channel belt is equivalent to the distribution of times to return to the origin, which has a right-hand tail that follows a power-law scaling with an exponent of -3/2. As such, the mean and variance of ages of sediment deposits in the channel belt do not converge to stable values over time but depend on the time since the formation of the channel belt. This result has implications for storage times and chemical alteration of floodplain sediments, as well as the interpretation of measured sediment ages. Model predictions compare well to data of sediment age distributions measured at field sites and the temporal evolution of channel belts observed in flume experiments. Both comparisons indicate that a random walk approach adequately describes the lateral migration of channels and the formation of channel belts. The theoretical description of the temporal evolution of channel-belt width developed herein can be used for predictions, for example, in hazard mitigation and stream restoration, and to invert fluvial strata for ambient hydraulic conditions. Further, it may serve to connect models designed for either geological or process timescales.

Background and Objectives

The pharmacokinetics (PK) of piperacillin/tazobactam (PIP/TAZ) is highly variable across different patient populations and there are controversies regarding non-linear elimination as well as the fraction unbound of PIP (fUNB_PIP). This has led to a plethora of subgroup-specific models, increasing the risk of misusing published models when optimising dosing regimens. In this study, we aimed to develop a single model to simultaneously describe the PK of PIP/TAZ in diverse patient populations and evaluate the current dosing recommendations by predicting the PK/pharmacodynamics (PD) target attainment throughout life.

Methods

Population PK models were separately built for PIP and TAZ based on data from 13 studies in various patient populations. In the development of those single-drug models, postnatal age (PNA), postmenstrual age (PMA), total body weight (TBW), height, and serum creatinine (SCR) were tested as covariates. Subsequently, a combined population PK model was established and the correlations between the PK of PIP and TAZ were tested. Monte Carlo simulations were performed based on the final combined model to evaluate the current dosing recommendations.

Results

The final combined model for PIP/TAZ consisted of four compartments (two for each drug), with covariates including TBW, PMA, and SCR. For a 70-kg, 35-year-old patient with SCR of 0.83 mg L−1, the PIP values for V1, CL, V2 and Q2 were 10.4 L, 10.6 L h−1, 11.6 L and 15.2 L h−1, respectively, and the TAZ values were 10.5 L, 9.58 L h−1, 13.7 L and 16.8 L h−1, respectively. The CL for both drugs show maturation in early life, reaching 50% at 54.2 weeks PMA. With advancing age, CL of TAZ declines to 50% at 61.6 years PMA, whereas CL of PIP declines more slowly, reaching 50% at 89.1 years PMA. The fUNB_PIP was estimated as 64.5% and non-linear elimination was not supported by our data. The simulation results indicated considerable differences in PK/PD target attainment for different patient populations under current recommended dosing regimens.

Conclusions

We developed a combined population PK model for PIP/TAZ across a broad range of patients covering the extremes of patient characteristics. This model can be used as a robust a priori model for Bayesian forecasting to achieve individualised dosing. The simulations indicate that adjustments based on the allometric theory as well as maturation and decline of CL of PIP may help the current dosing recommendations to provide consistent target attainment across patient populations.

Herein, we report the solvent-dependent reactivity of Fe(CO)5 toward AsF5 in either anhydrous HF or liquid SO2. The reaction of Fe(CO)5 with the superacid HF/AsF5 leads to the protonation of the iron center and allows for the first-time structural characterization of [FeH(CO)5]+ in the solid state, representing one of the most acidic transition metal hydride complexes to ever be isolated and structurally characterized. In the aprotic but oxidation-stable solvent SO2, Fe(CO)5 is oxidized and dimerized to [Fe2(CO)10]2+, which is isoelectronic with well-known Mn2(CO)10. [Fe2(CO)10]2+ is the first structurally characterized example of a homoleptic dinuclear transition metal carbonyl cation. Together with Fe(CO)5 and [Fe(CO)5]+•, it is a rare example of an iron-centered triad from which the neutral, the radical cationic, and the dimerized dicationic species have been structurally and spectroscopically characterized. All characterizations are well supported by quantum chemical calculations. We also make the argument that the dimerization of [Fe(CO)5]+• is largely dependent on the employed solvent.

Isomerization is a key process in many (bio)chemical systems. In microbial rhodopsins, the photoinduced isomerization of the all-trans retinal to the 13-cis isomer initiates a cascade of structural changes of the protein. The interplay between these changes and the thermal relaxation of the isomerized retinal is one of the crucial determinants for rhodopsin functionality. It is therefore important to probe this dynamic interplay with chromophore specific markers that combine gapless temporal observation with spectral sensitivity. Here we utilize the near-UV and mid-IR fingerprint region in the framework of a systematic (time-resolved) spectroscopic study on H+- (HsBR, (G)PR), Na+- (KR2, ErNaR) and Cl−-(NmHR) pumps. We demonstrate that the near-UV region is an excellent probe for retinal configuration and—being sensitive to the electrostatic environment of retinal—even transient ion binding, which allows us to pinpoint protein specific mechanistic nuances and chromophore-charge interactions. The combination of the near-UV and mid-IR fingerprint region hence provides a spectroscopic analysis tool that allows a detailed, precise and temporally fully resolved description of retinal configurations during all stages of the photocycle.

Following the current rise of cross-sector networks in education, we take a closer look at the supply side in the field of cultural education in Germany. We consider goal diversity in cross-sector collaborations and aim to provide insights into the group-specific goals of actors in the initial phase of collaborations. Using the lens of collaborative governance, collaborative engagement, and goal diversity research, we conducted 24 semi-structured interviews which we analyzed using thematic qualitative content analysis. We identified distinct goals for each of the five participating groups. These goals can be assigned to the macro, meso, and micro levels of the network, providing information about the direction of the goals and emphasizing the dynamic interplay of goals and their implications for collaborative dynamics. Future research could determine whether the results can be found in other contexts.

Interfaces in heterostructures play a major role in the functionality of electronic devices. Phenomena such as charge trapping/detrapping at interfaces under electric field affect the dynamics of metal/oxide/metal capacitors and metal/oxide/semiconductor transistors used for memory and logic applications. Charge traps are also key for the stabilization of a ferroelectric polarization and its ability to switch in ferroelectric devices such as ferroelectric tunnel junctions (FTJs). However, electric-field induced charging phenomena remain unclear even in conventional dielectric heterostructures due to a lack of direct measurement methods. Here, it is shown how operando off-axis electron holography can be used to quantify the charges trapped at the dielectric/dielectric interfaces as well as metal/dielectric interfaces in HfO2- and Al2O3-based nanocapacitors. By mapping the electrostatic potential at sub-nanometer spatial resolution while applying a bias, it is demonstrated that these interfaces present a high density of trapped charges, which strongly influence the electric field distribution within the device. The unprecedented sensitivity of the electron holography experiments coupled with numerical simulations highlights for the first time the linear relationship between the trapped charges at each interface and the applied bias, and the effect of the trapped charges on the local electrical behavior.

We develop an integrative conceptual framework and research agenda for studying epistemic authorities in the digital age. Consulting epistemic authorities (e.g., professional experts, well-informed laypeople, technologies) can be an efficient fast-track to knowledge. To fulfill this functional role, those who claim epistemic authority need to be both subjectively recognized (have a perceived advantage in knowledge) and objectively justified (have an actual advantage in knowledge). In a digital media context, new and unconventional knowledge sources have emerged that can fulfill the functional role of epistemic authorities. But false authorities that disseminate misinformation have emerged as well while other sources with important knowledge remain unrecognized. We further analyze the functional role of epistemic intermediaries that can mitigate such problematic developments by correcting false authorities and by providing endorsement for unrecognized authorities. We conclude with a research agenda to study functional forms of epistemic authorities and epistemic intermediaries in the digital public sphere.

Francisella tularensis, a category A bioterrorism agent, causes tularemia in many animal species. F. tularensis subspecies tularensis (type A) and holarctica (type B) are mainly responsible for human tularemia. The high mortality rate of 30–60 % caused by F. tularensis subspecies tularensis if left untreated and the aerosol dispersal renders this pathogen a dangerous bioagent. While a live attenuated vaccine strain (LVS) of F. tularensis type B does not provide sufficient protection against all forms of tularemia infections, a significant level of protection against F. tularensis has been observed for both passive and active immunization of mice with isolated O-antigen capsular polysaccharide. Well-defined, synthetic oligosaccharides offer an alternative approach towards the development of glycoconjugate vaccines. To identify diagnostics and therapeutics leads against tularemia, a collection of F. tularensis strain 15 O-antigen capsular polysaccharide epitopes were chemically synthesized. Glycan microarrays containing synthetic glycans were used to analyze the sera of tularemia-infected and non-infected animals and revealed the presence of IgG antibodies against the glycans. Two disaccharide (13 and 18), both bearing a unique formamido moiety, were identified as minimal glycan epitopes for antibody binding. These epitopes are the starting point for the development of diagnostics and therapeutics against tularemia.

Within the last two decades, the European Medicines Agency and the US Food and Drug Administration have approved several gene therapies. One category is oligonucleotide therapeutics, which allow for the regulation of the expression of target genes. Besides already approved therapeutics, there are several preclinical and clinical trials ongoing. The World Anti-Doping Agency prohibits the use of “nucleic acids or nucleic acid analogs that may alter genome sequences and/or alter gene expression by any mechanism” as a nonspecified method at all times. Hence, the administration of nucleic acids or analogs by athletes would cause an Anti-Doping Rule Violation. Herein, we discuss types of oligonucleotide therapeutics, their potential to be misused in sports, and considerations to sample preparation and mass spectrometric approaches with regard to antidoping analysis.

Oxo-graphene nanoribbons (oxo-GNRs) can be prepared by the oxidative unzipping of single-walled carbon nanotubes. We present an orthogonal functionalization method for the functionalization of the rims and the π-surface, respectively, what is only possible due to the high rim portion in oxo-GNRs. In particular, X-ray photoelectron spectroscopy could be used to follow the reaction and detect the marker atoms contained in the addends. We propose that the reported orthogonal functionalization strategy can also be applied on other oxo-functionalized carbon materials, such as graphene quantum dots, or reduced graphene oxide flakes.

This article aims to discuss the ethical–political responsibility of constructing a memory of 1915 through Fatih Akın’s The Cut (2014). The film explores the Armenian Genocide, which Turkey’s official historiography denies, and sparked heated debates on its release in Turkey. Based on the claim that constructing a memory of genocide is an ethical–political issue, I argue that The Cut’s aesthetic of remembrance fails to lead to an ethical questioning of historical denial and it thus leaves 1915 in the past. In order to discuss the film’s failure to fulfill its ethical responsibility, I explore the following questions: What is the ethical responsibility of remembering the devastating past? What does The Cut’s way of remembering 1915 accomplish and fail to accomplish in terms of ethical memory? By examining the limitations and possibilities of cinema in memory construction, this study seeks to contribute to discussions on the aesthetic and ethical dimensions of memory studies.

Scanning Transmission X-ray microscopy (STXM) is a sensitive and selective probe for the penetration of rapamycin which is topically applied to human skin ex vivo and is facilitated by skin treatment with microneedles puncturing the skin. Inner-shell excitation serves as a selective probe for detecting rapamycin by changes in optical density as well as linear combination modeling using reference spectra of the most abundant species. The results indicate that mechanical damage induced by microneedles allows this drug to accumulate in the stratum corneum without reaching the viable skin layers. This is unlike intact skin which shows no drug penetration at all and underscores the mechanical impact of microneedle skin treatment. These results are compared to drug penetration profiles of other drugs highlighting the importance of skin barriers. High spatial resolution studies also indicate that the lipophilic drug rapamycin is observed in corneocytes. Attempts in data evaluation are reported to probe rapamycin also in the lipid layers between the corneocytes, which was not accomplished before. These results are compared to recent results on rapamycin uptake in skin where barrier impairment was induced by pre-treatment with the enzyme trypsin and drug formulations leading to occlusion.

Background

Haptoglobin (Hp) is an emerging diagnostic marker in cattle, and knowledge of suitable sample types and measurement methods is important.

Objectives

The aims of this study were to compare the results of a colorimetric assay (CA) and an ELISA for bovine Hp using serum, EDTA plasma, and lithium-heparinized (LH) plasma, respectively, and to assess the diagnostic potential for puerperal metritis.

Methods

In experiment 1, Hp was measured in pooled aliquots of serum (n = 10), EDTA plasma (n = 10), and LH plasma (n = 10) of 100 healthy fresh lactating dairy cows from 10 farms using both the CA and the ELISA. In experiment 2, five healthy and five cows with acute puerperal metritis were sampled, and Hp was determined using both assays for all three sample types. In experiment 3, aliquots of serum and LH plasma from cows in different lactation stages were transferred into plain, EDTA-coated, and LH-coated tubes and mixed before colorimetric analyses. Distilled water was also placed into each tube type and treated similarly.

Results

Plasma samples measured with the CA showed on average 2.3 (EDTA) and 2.5 (LH) times higher Hp concentrations compared with serum, whereas no differences were seen with the ELISA results between sample types. Based on a clinical cut-off value, both methods differentiated sick from healthy cows. Haptoglobin measurements with the ELISA were less precise compared with CA measurements due to high dilutions. No influence of the anticoagulants on the CA was observed.

Conclusions

Due to measurement discrepancies between serum and plasma, CAs for bovine Hp based on peroxidase activity should be performed with serum, or specific reference ranges for plasma samples should be established. In this study, CA results obtained with LH plasma were more precise than results obtained with EDTA plasma. Both the CA and the ELISA are suitable diagnostic tools for the diagnosis of puerperal metritis, but CA measurements were more precise in this study.

Conflict-related sexual violence (CRSV) can severely impair survivors’ mental health and prompt their need for mental health care. Male survivors face gender-related barriers to disclosing experiences of sexual violence, yet the understanding of factors impeding or promoting disclosure is limited. This knowledge gap is even more pronounced regarding male disclosure in refugee settings. The high prevalence of CRSV experiences in male refugees in European host countries points to the urgency of understanding male disclosure processes along with its barriers and facilitators, in order to provide for adequate mental health care. 10 clinical experts working with male survivors of CRSV in Germany were interviewed adopting an explorative phenomenological approach and using the Problem-Centered Interview. Qualitative content analysis yielded five themes describing male disclosure of CRSV: 1. Experiences of CRSV commonly remain nonverbalized, 2. Disclosure of CRSV is a dialogical and iterative process, 3. The process of (non-)disclosure is a negotiation of agency, 4. Disclosure of CRSV affects the survivor’s psychoemotional state, and 5. Disclosure and recovery are interdependent processes. Additionally, seven categories describing barriers to and facilitators of male disclosure were extracted: 1. trauma characteristics, 2. survivor variables, 3. clinician variables, 4. interpreter variables, 5. interpersonal variables, 6. contextual variables, and 7. sociocultural variables. Clinical experts emphasize the effectivity of a gender-specific communicative taboo for male refugee survivors that intersect with socio-cultural norms. Variables characterizing survivors who are at risk of not disclosing CRSV are identified. A discussion of clinical approaches to address the taboo surrounding CRSV and to support male refugee survivors in agentic disclosure and recovery is provided.

The article examines varieties of trade union protest across industrial relations regimes, using protest event data for 27 European countries between 2000 and 2021. We present a large-n analysis of how the level and ‘movement character’ of union protest covaries with the strength and institutional settings of union movements across regimes. We show that unions remain important protest actors and that union protest in the public sphere notably outweighs workplace-related strikes. Furthermore, we find an inverse relationship between union institutionalization and the ‘movement character’ of union protest: While strong union movements in highly institutionalized regimes display a strike-heavy repertoire, weaker union movements in contexts of low institutionalization rely heavily on protest actions beyond the workplace strike. With these findings, we provide a novel empirical assessment of what unions do in the protest arena and how institutional settings can be conducive to strike-heavy versus protest-heavy union tactics.

In Nature, most known objects can perform their functions only when in supramolecular self-assembled from, e.g. protein complexes and cell membranes. Here, a dendritic polymer is presented that inhibits severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) with an irreversible (virucidal) mechanism only when self-assembled into a Two-dimmensional supramolecular polymer (2D-SupraPol). Monomeric analogs of the dendritic polymer can only inhibit SARS-CoV-2 reversibly, thus allowing for the virus to regain infectivity after dilution. Upon assembly, 2D-SupraPol shows a remarkable half-inhibitory concentration (IC50 30 nM) in vitro and in vivo in a Syrian Hamster model has a good efficacy. Using cryo-TEM, it is shown that the 2D-SupraPol has a controllable lateral size that can be tuned by adjusting the pH and use small angle X-ray and neutron scattering to unveil the architecture of the supramolecular assembly. This functional 2D-SupraPol, and its supramolecular architecture are proposed, as a prophylaxis nasal spray to inhibit the virus interaction with the respiratory tract.

This research sheds light on two crucial yet overlooked aspects of work interruptions: the perspective of employees who initiate interruptions and the reasons behind those interruptions. Building on earlier research on interruptions and theories on employee motivation, we identified six key reasons for interruptions that we integrated into a typology. This typology combined three interruption topics (performance, belongingness, and hedonic well-being) and two interruption foci (benefitting the interrupter and benefitting the interruptee; i.e., self-focused and other-focused). We validated this typology using qualitative reports and a scale-development approach, thereby creating the interruption-for-a-reason scale (IFRS). We found that interruptions were typically initiated for good reasons and positively correlated with social exchange constructs. That is, initiating interruptions was linked to requesting social support and to performing prosocial behaviors to coworkers. Altogether, this research contributes to a more comprehensive understanding of interruptions by offering a new perspective on interruptions that addresses the complexities of this phenomenon. Illuminating the interrupters' perspective and the various reasons for interruptions is key to a more balanced examination of the positive and negative aspects of interruptions.