dc.contributor.author
Tsvetkov, Dmitry
dc.contributor.author
Tano, Jean-Yves
dc.contributor.author
Kassmann, Mario
dc.contributor.author
Wang, Ning
dc.contributor.author
Schubert, Rudolf
dc.contributor.author
Gollasch, Maik
dc.date.accessioned
2018-06-08T04:17:42Z
dc.date.available
2016-09-16T11:13:27.789Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/16988
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-21168
dc.description.abstract
The anti-contractile effect of perivascular adipose tissue (PVAT) is an
important mechanism in the modulation of vascular tone in peripheral arteries.
Recent evidence has implicated the XE991-sensitive voltage-gated KV (KCNQ)
channels in the regulation of arterial tone by PVAT. However, until now the in
vivo pharmacology of the involved vascular KV channels with regard to XE991
remains undetermined, since XE991 effects may involve Ca2+ activated BKCa
channels and/or voltage-dependent KV1.5 channels sensitive to diphenyl
phosphine oxide-1 (DPO-1). In this study, we tested whether KV1.5 channels are
involved in the control of mesenteric arterial tone and its regulation by
PVAT. Our study was also aimed at extending our current knowledge on the in
situ vascular pharmacology of DPO-1 and XE991 regarding KV1.5 and BKCa
channels, in helping to identify the nature of K+ channels that could
contribute to PVAT-mediated relaxation. XE991 at 30 μM reduced the anti-
contractile response of PVAT, but had no effects on vasocontraction induced by
phenylephrine (PE) in the absence of PVAT. Similar effects were observed for
XE991 at 0.3 μM, which is known to almost completely inhibit mesenteric artery
VSMC KV currents. 30 μM XE991 did not affect BKCa currents in VSMCs. Kcna5−/−
arteries and wild-type arteries incubated with 1 μM DPO-1 showed normal
vasocontractions in response to PE in the presence and absence of PVAT. KV
current density and inhibition by 30 μM XE991 were normal in mesenteric artery
VSMCs isolated from Kcna5−/− mice. We conclude that KV channels are involved
in the control of arterial vascular tone by PVAT. These channels are present
in VSMCs and very potently inhibited by the KCNQ channel blocker XE991. BKCa
channels and/or DPO-1 sensitive KV1.5 channels in VSMCs are not the downstream
mediators of the XE991 effects on PVAT-dependent arterial vasorelaxation.
Further studies will need to be undertaken to examine the role of other KV
channels in the phenomenon.
en
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
KV1.5 channels
dc.subject
adipocyte-derived relaxing factor (ADRF)
dc.subject
perivascular adipose tissue (PVAT)
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit
dc.title
The Role of DPO-1 and XE991-Sensitive Potassium Channels in Perivascular
Adipose Tissue-Mediated Regulation of Vascular Tone
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Front. Physiol. - 7 (2016), Artikel Nr. 335
dcterms.bibliographicCitation.doi
10.3389/fphys.2016.00335
dcterms.bibliographicCitation.url
http://dx.doi.org/10.3389/fphys.2016.00335
refubium.affiliation
Charité - Universitätsmedizin Berlin
de
refubium.mycore.fudocsId
FUDOCS_document_000000025374
refubium.note.author
Der Artikel wurde in einer Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000006964
dcterms.accessRights.openaire
open access