dc.contributor.author
Hosaka, Hitomi
dc.contributor.author
Haruki, Risa
dc.contributor.author
Yamada, Kana
dc.contributor.author
Böttcher, Christoph
dc.contributor.author
Komatsu, Teruyuki
dc.date.accessioned
2018-06-08T03:53:15Z
dc.date.available
2014-11-06T12:05:45.281Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/16145
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-20329
dc.description.abstract
A covalent core–shell structured protein cluster composed of hemoglobin (Hb)
at the center and human serum albumins (HSA) at the periphery, Hb-HSAm, is an
artificial O2 carrier that can function as a red blood cell substitute. Here
we described the preparation of a novel Hb-HSA3 cluster with antioxidant
activities and its O2 complex stable in aqueous H2O2 solution. We used an
approach of incorporating a Pt nanoparticle (PtNP) into the exterior HSA unit
of the cluster. A citrate reduced PtNP (1.8 nm diameter) was bound tightly
within the cleft of free HSA with a binding constant (K) of 1.1×107 M−1,
generating a stable HSA-PtNP complex. This platinated protein showed high
catalytic activities for dismutations of superoxide radical anions (O2•–) and
hydrogen peroxide (H2O2), i.e., superoxide dismutase and catalase activities.
Also, Hb-HSA3 captured PtNP into the external albumin unit (K = 1.1×107 M−1),
yielding an Hb-HSA3(PtNP) cluster. The association of PtNP caused no
alteration of the protein surface net charge and O2 binding affinity. The
peripheral HSA-PtNP shell prevents oxidation of the core Hb, which enables the
formation of an extremely stable O2 complex, even in H2O2 solution.
en
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject.ddc
500 Naturwissenschaften und Mathematik::540 Chemie
dc.title
Hemoglobin–Albumin Cluster Incorporating a Pt Nanoparticle
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
PLoS ONE. - 9 (2014), 10, Artikel Nr. e110541
dc.title.subtitle
Artificial O2 Carrier with Antioxidant Activities
dcterms.bibliographicCitation.doi
10.1371/journal.pone.0110541
dcterms.bibliographicCitation.url
http://dx.plos.org/10.1371/journal.pone.0110541
refubium.affiliation
Biologie, Chemie, Pharmazie
de
refubium.mycore.fudocsId
FUDOCS_document_000000021249
refubium.note.author
Der Artikel wurde in einer Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000004115
dcterms.accessRights.openaire
open access