dc.contributor.author
Wächter, Miriam
dc.contributor.author
Wölfel, Silke
dc.contributor.author
Pfeffer, Martin
dc.contributor.author
Dobler, Gerhard
dc.contributor.author
Kohn, Barbara
dc.contributor.author
Moritz, Andreas
dc.contributor.author
Pachnicke, Stefan
dc.contributor.author
Silaghi, Cornelia
dc.date.accessioned
2018-06-08T03:50:14Z
dc.date.available
2015-04-22T06:12:00.777Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/16036
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-20222
dc.description.abstract
Background Spotted Fever Group (SFG) Rickettsiae can cause febrile diseases
with or without rash in humans worldwide. In Germany only limited data are
available about their medical significance. Serological screening tests for
antibodies against rickettsiae usually only distinguish between SFG and Typhus
Group (TG) Rickettsiae due to the strong cross reactivities within the groups.
Seroprevalence rates in dogs, as possible sentinels for tick-borne diseases,
could serve as an indicator for the distribution of different Rickettsia
species. Methods In this study, a micro-immunofluorescence assay (micro-IFA)
was established for detection and differentiation of antibodies against five
Rickettsia species in dogs (R. helvetica, R. raoultii, R. slovaca, R.
monacensis and R. felis). Dogs that never left Germany (n = 605) previously
investigated with an SFG-ELISA were included in this study and screened at a
1:128 dilution. Endpoint titres of fifty randomly selected seropositive
samples of each of the five investigated regions in Germany were determined in
order to allow a differentiation of the causative Rickettsia species.
Sensitivity and specificity of the micro-IFA were compared with ELISA results
of the previous study. Results A total of 93.9% of the dogs were positive for
antibodies of the SFG Rickettsiae at the screening titer of 1:128.
Differentiation of SFG Rickettsiae with the micro-IFA was possible in 70.4%,
but in 29.6% of the cases the detected antibodies were not differentiable.
Considering a clear differentiation by a twofold titre difference between
observed reactions, the seroprevalence rates were 66.0% for R. helvetica, 2.8%
for R. raoultii, 1.6% for R. slovaca, but no serological reaction could be
clearly attributed to R. monacensis or R. felis. No statistically significant
regional differences were found for R. helvetica, R. slovaca and R. raoultii
comparing the five regions of Germany. Comparison of micro-IFA with ELISA
revealed a sensitivity of 82.0% and a specificity of 83.8% for the Rickettsia
SFG ELISA. Conclusions The micro-IFA is a useful serological tool to
differentiate antibodies against different Rickettsia species in dogs.
Seroprevalence rates in dogs correspond to the prevalence rates and
distribution of Rickettsia-carrying tick species.
de
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft
dc.title
Serological differentiation of antibodies against Rickettsia helvetica, R.
raoultii, R. slovaca, R. monacensis and R. felis in dogs from Germany by a
micro-immunofluorescent antibody test
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Parasites & Vectors. - 8 (2015), 1, Artikel Nr. 126
dcterms.bibliographicCitation.doi
10.1186/s13071-015-0745-1
dcterms.bibliographicCitation.url
http://www.parasitesandvectors.com/content/8/1/126
refubium.affiliation
Veterinärmedizin
de
refubium.mycore.fudocsId
FUDOCS_document_000000022247
refubium.note.author
Der Artikel wurde in einer Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000004794
dcterms.accessRights.openaire
open access