dc.contributor.author
Tsangaras, Kyriakos
dc.contributor.author
Mayer, Jens
dc.contributor.author
Alquezar-Planas, David E.
dc.contributor.author
Greenwood, Alex D.
dc.date.accessioned
2018-06-08T03:49:50Z
dc.date.available
2016-01-07T12:07:54.570Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/16012
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-20198
dc.description.abstract
Transcriptome analysis of polar bear (Ursus maritimus) tissues identified
sequences with similarity to Porcine Endogenous Retroviruses (PERV). Based on
these sequences, four proviral copies and 15 solo long terminal repeats (LTRs)
of a newly described endogenous retrovirus were characterized from the polar
bear draft genome sequence. Closely related sequences were identified by PCR
analysis of brown bear (Ursus arctos) and black bear (Ursus americanus) but
were absent in non-Ursinae bear species. The virus was therefore designated
UrsusERV. Two distinct groups of LTRs were observed including a recombinant
ERV that contained one LTR belonging to each group indicating that genomic
invasions by at least two UrsusERV variants have recently occurred. Age
estimates based on proviral LTR divergence and conservation of integration
sites among ursids suggest the viral group is only a few million years old.
The youngest provirus was polar bear specific, had intact open reading frames
(ORFs) and could potentially encode functional proteins. Phylogenetic analyses
of UrsusERV consensus protein sequences suggest that it is part of a pig,
gibbon and koala retrovirus clade. The young age estimates and lineage
specificity of the virus suggests UrsusERV is a recent cross species
transmission from an unknown reservoir and places the viral group among the
youngest of ERVs identified in mammals.
en
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
endogenous retrovirus
dc.subject
next generation sequencing
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft
dc.title
An Evolutionarily Young Polar Bear (Ursus maritimus) Endogenous Retrovirus
Identified from Next Generation Sequence Data
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Viruses. - 7 (2015), 11, S. 6089-6107
dcterms.bibliographicCitation.doi
10.3390/v7112927
dcterms.bibliographicCitation.url
http://www.mdpi.com/1999-4915/7/11/2927
refubium.affiliation
Veterinärmedizin
de
refubium.mycore.fudocsId
FUDOCS_document_000000023692
refubium.note.author
Der Artikel wurde in einer Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000005827
dcterms.accessRights.openaire
open access