dc.contributor.author
Repen, Boris
dc.contributor.author
Schneider, Erwin
dc.contributor.author
Alexiev, Ulrike
dc.date.accessioned
2018-06-08T03:37:34Z
dc.date.available
2014-06-02T10:23:38.793Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/15586
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-19774
dc.description.abstract
We studied the activity of the fluorescently labeled membrane transporter
MalGFK2, which transports maltose at the expense of ATP hydrolysis. We used a
commercially available malachite green assay (SensoLyte MG phosphate assay
kit; Anaspec) to quantify the liberated phosphate upon ATP hydrolysis.
However, strong variations in phosphate concentration were measured when using
the supplier’s handling protocol. We optimized the protocol, taking into
account the effects mediated by glycerol, SDS, and fluorescent label on the
sample. As a result we obtained highly reproducible phosphate concentration
values under conditions optimal for solubilized membrane proteins.
de
dc.rights.uri
http://www.elsevier.com/wps/find/authorsview.authors/authorsrights
dc.subject
Inorganic phosphate detection
dc.subject
Malachite green
dc.subject
Integral membrane proteins
dc.subject
Enzymatic ATP hydrolysis
dc.subject
ABC transporter
dc.subject
Fluorescent reporter groups
dc.subject.ddc
500 Naturwissenschaften und Mathematik::530 Physik
dc.title
Optimization of a malachite green assay for detection of ATP hydrolysis by
solubilized membrane proteins
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Analytical Biochemistry. - 426 (2012), 2, S.103-105
dc.identifier.sepid
33054
dcterms.bibliographicCitation.doi
10.1016/j.ab.2012.04.016
dcterms.bibliographicCitation.doi
10.1016/j.ab.2012.04.016
dcterms.bibliographicCitation.url
http://linkinghub.elsevier.com/retrieve/pii/S000326971200228X
refubium.affiliation
Physik
de
refubium.affiliation.other
Institut für Experimentalphysik
refubium.mycore.fudocsId
FUDOCS_document_000000020435
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000003619
dcterms.accessRights.openaire
open access
dcterms.isPartOf.issn
00032697
