dc.contributor.author
Jouhanneau, Jean-Sébastien
dc.contributor.author
Kremkow, Jens
dc.contributor.author
Dorrn, Anja L.
dc.contributor.author
Poulet, James F.A.
dc.date.accessioned
2018-06-08T03:27:42Z
dc.date.available
2016-01-28T12:42:06.735Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/15228
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-19416
dc.description.abstract
Little is known about the properties of monosynaptic connections between
identified neurons in vivo. We made multiple (two to four) two-photon targeted
whole-cell recordings from neighboring layer 2 mouse somatosensory barrel
cortex pyramidal neurons in vivo to investigate excitatory monosynaptic
transmission in the hyperpolarized downstate. We report that pyramidal neurons
form a sparsely connected (6.7% connectivity) network with an
overrepresentation of bidirectional connections. The majority of unitary
excitatory postsynaptic potentials were small in amplitude (<0.5 mV), with a
small minority >1 mV. The coefficient of variation (CV = 0.74) could largely
be explained by the presence of synaptic failures (22%). Both the CV and
failure rates were reduced with increasing amplitude. The mean paired-pulse
ratio was 1.15 and positively correlated with the CV. Our approach will help
bridge the gap between connectivity and function and allow investigations into
the impact of brain state on monosynaptic transmission and integration.
en
dc.rights.uri
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit
dc.title
In Vivo Monosynaptic Excitatory Transmission between Layer 2 Cortical
Pyramidal Neurons
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Cell Reports. - 13 (2015) 10, S. 2098-2106
dcterms.bibliographicCitation.doi
10.1016/j.celrep.2015.11.011
dcterms.bibliographicCitation.url
http://www.sciencedirect.com/science/article/pii/S221112471501311X
refubium.affiliation
Charité - Universitätsmedizin Berlin
de
refubium.mycore.fudocsId
FUDOCS_document_000000023817
refubium.note.author
Der Artikel wurde in einer Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000005924
dcterms.accessRights.openaire
open access