dc.contributor.author
Hertlein, Gillian
dc.contributor.author
Müller, Sebastian
dc.contributor.author
Garcia-Gonzalez, Eva
dc.contributor.author
Poppinga, Lena
dc.contributor.author
Süssmuth, Roderich D.
dc.contributor.author
Genersch, Elke
dc.date.accessioned
2018-06-08T03:24:42Z
dc.date.available
2014-12-17T13:04:43.388Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/15111
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-19299
dc.description.abstract
The Gram-positive bacterium Paenibacillus larvae is the etiological agent of
American Foulbrood. This bacterial infection of honey bee brood is a
notifiable epizootic posing a serious threat to global honey bee health
because not only individual larvae but also entire colonies succumb to the
disease. In the recent past considerable progress has been made in elucidating
molecular aspects of host pathogen interactions during pathogenesis of P.
larvae infections. Especially the sequencing and annotation of the complete
genome of P. larvae was a major step forward and revealed the existence of
several giant gene clusters coding for non-ribosomal peptide synthetases which
might act as putative virulence factors. We here present the detailed analysis
of one of these clusters which we demonstrated to be responsible for the
biosynthesis of bacillibactin, a P. larvae siderophore. We first established
culture conditions allowing the growth of P. larvae under iron-limited
conditions and triggering siderophore production by P. larvae. Using a gene
disruption strategy we linked siderophore production to the expression of an
uninterrupted bacillibactin gene cluster. In silico analysis predicted the
structure of a trimeric trithreonyl lactone (DHB-Gly-Thr)3 similar to the
structure of bacillibactin produced by several Bacillus species. Mass
spectrometric analysis unambiguously confirmed that the siderophore produced
by P. larvae is identical to bacillibactin. Exposure bioassays demonstrated
that P. larvae bacillibactin is not required for full virulence of P. larvae
in laboratory exposure bioassays. This observation is consistent with results
obtained for bacillibactin in other pathogenic bacteria.
en
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Production of the Catechol Type Siderophore Bacillibactin by the Honey Bee
Pathogen Paenibacillus larvae
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
PLoS ONE. - 9 (2014), 9, Art.-Nr. e108272
dc.identifier.sepid
39653
dcterms.bibliographicCitation.doi
10.1371/journal.pone.0108272
dcterms.bibliographicCitation.url
http://dx.doi.org/10.1371/journal.pone.0108272
refubium.affiliation
Veterinärmedizin
de
refubium.affiliation.other
Institut für Mikrobiologie und Tierseuchen
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refubium.mycore.fudocsId
FUDOCS_document_000000021480
refubium.note.author
Der Artikel wurde in einer Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000004294
dcterms.accessRights.openaire
open access
dcterms.isPartOf.issn
1932-6203