dc.contributor.author
Zimmermann, Jonas
dc.contributor.author
Abarca, Nelida
dc.contributor.author
Enk, Neela
dc.contributor.author
Skibbe, Oliver
dc.contributor.author
Kusber, Wolf-Henning
dc.contributor.author
Jahn, Regine
dc.date.accessioned
2018-06-08T03:22:01Z
dc.date.available
2015-01-12T14:10:51.548Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/15014
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-19202
dc.description.abstract
DNA barcoding uses a short fragment of a DNA sequence to identify a taxon.
After obtaining the target sequence it is compared to reference sequences
stored in a database to assign an organism name to it. The quality of data in
the reference database is the key to the success of the analysis. In the here
presented study, multiple types of data have been combined and critically
examined in order to create best practice guidelines for taxonomic reference
libraries for environmental barcoding. 70 unialgal diatom strains from Berlin
waters have been established and cultured to obtain morphological and
molecular data. The strains were sequenced for 18S V4 rDNA (the pre-Barcode
for protists) as well as rbcL data, and identified by microscopy. LM and for
some strains also SEM pictures were taken and physical vouchers deposited at
the BGBM. 37 freshwater taxa from 15 naviculoid diatom genera were identified.
Four taxa from the genera Amphora, Mayamaea, Planothidium and Stauroneis are
described here as new. Names, molecular, morphological and habitat data as
well as additional images of living cells are also available electronically in
the AlgaTerra Information System. All reference sequences (or reference
barcodes) presented here are linked to voucher specimens in order to provide a
complete chain of evidence back to the formal taxonomic literature.
en
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject.ddc
500 Naturwissenschaften und Mathematik::580 Pflanzen (Botanik)
dc.title
Taxonomic Reference Libraries for Environmental Barcoding
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
PLoS ONE. - 9 (2014), 9, Artikel Nr. e108793
dc.title.subtitle
A Best Practice Example from Diatom Research
dcterms.bibliographicCitation.doi
10.1371/journal.pone.0108793
dcterms.bibliographicCitation.url
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0108793
refubium.affiliation
Botanischer Garten und Botanisches Museum Berlin-Dahlem (BGBM)
de
refubium.mycore.fudocsId
FUDOCS_document_000000021566
refubium.note.author
Der Artikel wurde in einer Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000004354
dcterms.accessRights.openaire
open access