dc.contributor.author
Gödde, Kathrin
dc.contributor.author
Gschwend, Olivier
dc.contributor.author
Puchkov, Dmytro
dc.contributor.author
Pfeffer, Carsten K.
dc.contributor.author
Carleton, Alan
dc.contributor.author
Jentsch, Thomas J.
dc.date.accessioned
2018-06-08T03:16:34Z
dc.date.available
2016-10-19T08:59:17.573Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/14828
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-19017
dc.description.abstract
Synaptic inhibition in the olfactory bulb (OB), the first relay station of
olfactory information, is believed to be important for odour discrimination.
We interfered with GABAergic inhibition of mitral and tufted cells (M/T
cells), the principal neurons of the OB, by disrupting their potassium-
chloride cotransporter 2 (Kcc2). Roughly, 70% of mice died around 3 weeks, but
surviving mice appeared normal. In these mice, the resulting increase in the
intracellular Cl− concentration nearly abolished GABA-induced
hyperpolarization of mitral cells (MCs) and unexpectedly increased the number
of perisomatic synapses on MCs. In vivo analysis of odorant-induced OB
electrical activity revealed increased M/T cell firing rate, altered phasing
of action potentials in the breath cycle and disrupted separation of odour-
induced M/T cell activity patterns. Mice also demonstrated a severely impaired
ability to discriminate chemically similar odorants or odorant mixtures. Our
work suggests that precisely tuned GABAergic inhibition onto M/T cells is
crucial for M/T cell spike pattern separation needed to distinguish closely
similar odours.
de
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Olfactory bulb
dc.subject
Sensory processing
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::611 Menschliche Anatomie, Zytologie, Histologie
dc.title
Disruption of Kcc2-dependent inhibition of olfactory bulb output neurons
suggests its importance in odour discrimination
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Nature Communications. - 7 (2016), Artikel Nr. 12043
dcterms.bibliographicCitation.doi
10.1038/ncomms12043
dcterms.bibliographicCitation.url
http://www.nature.com/articles/ncomms12043
refubium.affiliation
Charité - Universitätsmedizin Berlin
de
refubium.mycore.fudocsId
FUDOCS_document_000000025575
refubium.note.author
Der Artikel wurde in einer reinen Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000007235
dcterms.accessRights.openaire
open access