dc.contributor.author
Miessen, Katrin
dc.contributor.author
Einspanier, Ralf
dc.contributor.author
Schoen, Jennifer
dc.date.accessioned
2018-06-08T03:13:10Z
dc.date.available
2012-10-30T09:02:50.090Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/14703
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-18893
dc.description.abstract
Background Cervical uterine epithelial cells maintain a physiological and
pathogen-free milieu in the female mammalian reproductive tract and are
involved in sperm-epithelium interaction. Easily accessible, differentiated
model systems of the cervical epithelium are not yet available to elucidate
the underlying molecular mechanisms within these highly specialized cells.
Therefore, the aim of the study was to establish a cell culture of the porcine
cervical epithelium representing in vivo-like properties of the tissue.
Results We tested different isolation methods and culture conditions and
validated purity of the cultured cells by immunohistochemistry against
keratins. We could reproducibly culture pure epithelial cells from cervical
tissue explants. Based on a morphology score and the WST-1 Proliferation
Assay, we optimized the growth medium composition. Primary porcine cervical
cells performed best in conditioned Ham's F-12, containing 10% FCS, EGF and
insulin. After cultivation in an air-liquid interface for three weeks, the
cells showed a discontinuously multilayered phenotype. Finally,
differentiation was validated via immunohistochemistry against beta catenin.
Mucopolysaccharide production could be shown via alcian blue staining.
Conclusions We provide the first suitable protocol to establish a
differentiated porcine epithelial model of the cervix uteri, based on easily
accessible cells using slaughterhouse material.
de
dc.rights.uri
http://creativecommons.org/licenses/by/2.0/
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche
dc.title
Establishment and characterization of a differentiated epithelial cell culture
model derived from the porcine cervix uteri
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
BMC Veterinary Research 2012, 8:31
dcterms.bibliographicCitation.doi
10.1186/1746-6148-8-31
dcterms.bibliographicCitation.url
http://www.biomedcentral.com/1746-6148/8/31
refubium.affiliation
Veterinärmedizin
de
refubium.affiliation.other
Institut für Veterinär-Biochemie
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refubium.mycore.fudocsId
FUDOCS_document_000000014772
refubium.note.author
Gefördert durch die DFG und den Open Access Publikationsfonds der Freien
Universität Berlin
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000002114
dcterms.accessRights.openaire
open access