dc.contributor.author
Mühlhaus, Jessica
dc.contributor.author
Dinter, Juliane
dc.contributor.author
Nürnberg, Daniela
dc.contributor.author
Rehders, Maren
dc.contributor.author
Depke, Maren
dc.contributor.author
Golchert, Janine
dc.contributor.author
Homuth, Georg
dc.contributor.author
Yi, Chun-Xia
dc.contributor.author
Morin, Silke
dc.contributor.author
Köhrle, Josef
dc.contributor.author
Brix, Klaudia
dc.contributor.author
Tschöp, Matthias
dc.contributor.author
Kleinau, Gunnar
dc.contributor.author
Biebermann, Heike
dc.date.accessioned
2018-06-08T03:08:58Z
dc.date.available
2015-01-12T13:45:09.174Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/14581
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-18773
dc.description.abstract
The thyroid hormone derivative 3-iodothyronamine (3-T1AM) exerts metabolic
effects in vivo that contradict known effects of thyroid hormones. 3-T1AM acts
as a trace amine-associated receptor 1 (TAAR1) agonist and activates Gs
signaling in vitro. Interestingly, 3-T1AM-meditated in vivo effects persist in
Taar1 knockout-mice indicating that further targets of 3-T1AM might exist.
Here, we investigated another member of the TAAR family, the only scarcely
studied mouse and human trace-amine-associated receptor 8 (Taar8b, TAAR8). By
RT-qPCR and locked-nucleic-acid (LNA) in situ hybridization, Taar8b expression
in different mouse tissues was analyzed. Functionally, we characterized TAAR8
and Taar8b with regard to cell surface expression and signaling via different
G-protein-mediated pathways. Cell surface expression was verified by ELISA,
and cAMP accumulation was quantified by AlphaScreen for detection of Gs and/or
Gi/o signaling. Activation of G-proteins Gq/11 and G12/13 was analyzed by
reporter gene assays. Expression analyses revealed at most marginal Taar8b
expression and no gender differences for almost all analyzed tissues. In
heart, LNA-in situ hybridization demonstrated the absence of Taar8b
expression. We could not identify 3-T1AM as a ligand for TAAR8 and Taar8b, but
both receptors were characterized by a basal Gi/o signaling activity, a so far
unknown signaling pathway for TAARs.
en
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit
dc.title
Analysis of Human TAAR8 and Murine Taar8b Mediated Signaling Pathways and
Expression Profile
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
International Journal of Molecular Sciences. - 15 (2014), 11, S. 20638-20655
dcterms.bibliographicCitation.doi
10.3390/ijms151120638
dcterms.bibliographicCitation.url
http://www.mdpi.com/1422-0067/15/11/20638
refubium.affiliation
Charité - Universitätsmedizin Berlin
de
refubium.mycore.fudocsId
FUDOCS_document_000000021565
refubium.note.author
Der Artikel wurde in einer Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000004353
dcterms.accessRights.openaire
open access