dc.contributor.author
Hackstein, Holger
dc.contributor.author
Kranz, Sabine
dc.contributor.author
Lippitsch, Anne
dc.contributor.author
Wachtendorf, Andreas
dc.contributor.author
Kershaw, Olivia
dc.contributor.author
Gruber, Achim Dieter
dc.contributor.author
Michel, Gabriela
dc.contributor.author
Lohmeyer, Jürgen
dc.contributor.author
Bein, Gregor
dc.contributor.author
Baal, Nelli
dc.contributor.author
Herold, Susanne
dc.date.accessioned
2018-06-08T03:08:35Z
dc.date.available
2015-06-18T06:23:56.375Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/14570
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-18762
dc.description.abstract
Klebsiella pneumoniae is a leading cause of severe hospital-acquired
respiratory tract infections and death but little is known regarding the
modulation of respiratory dendritic cell (DC) subsets. Plasmacytoid DC (pDC)
are specialized type 1 interferon producing cells and considered to be
classical mediators of antiviral immunity. By using multiparameter flow
cytometry analysis we have analysed the modulation of respiratory DC subsets
after intratracheal Klebsiella pneumonia infection. Data indicate that pDCs
and MoDC were markedly elevated in the post acute pneumonia phase when
compared to mock-infected controls. Analysis of draining mediastinal lymph
nodes revealed a rapid increase of activated CD103+ DC, CD11b+ DC and MoDC
within 48 h post infection. Lung pDC identification during bacterial pneumonia
was confirmed by extended phenotyping for 120G8, mPDCA-1 and Siglec-H
expression and by demonstration of high Interferon-alpha producing capacity
after cell sorting. Cytokine expression analysis of ex vivo-sorted respiratory
DC subpopulations from infected animals revealed elevated Interferon-alpha in
pDC, elevated IFN-gamma, IL-4 and IL-13 in CD103+ DC and IL-19 and IL-12p35 in
CD11b+ DC subsets in comparison to CD11c+ MHC-class IIlow cells indicating
distinct functional roles. Antigen-specific naive CD4+ T cell stimulatory
capacity of purified respiratory DC subsets was analysed in a model system
with purified ovalbumin T cell receptor transgenic naive CD4+ responder T
cells and respiratory DC subsets, pulsed with ovalbumin and matured with
Klebsiella pneumoniae lysate. CD103+ DC and CD11b+ DC subsets represented the
most potent naive CD4+ T helper cell activators. These results provide novel
insight into the activation of respiratory DC subsets during Klebsiella
pneumonia infection. The detection of increased respiratory pDC numbers in
bacterial pneumonia may indicate possible novel pDC functions with respect to
lung repair and regeneration.
en
dc.rights.uri
http://creativecommons.org/licenses/by/2.0/
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft
dc.title
Modulation of respiratory dendritic cells during Klebsiella pneumonia
infection
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Respiratory Research. - 14 (2013), 1, Artikel Nr. 91
dc.identifier.sepid
33495
dcterms.bibliographicCitation.doi
10.1186/1465-9921-14-91
dcterms.bibliographicCitation.url
http://dx.doi.org/10.1186/1465-9921-14-91
refubium.affiliation
Veterinärmedizin
de
refubium.affiliation.other
Institut für Tierpathologie
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refubium.mycore.fudocsId
FUDOCS_document_000000022510
refubium.note.author
Der Artikel wurde in einer Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000005087
dcterms.accessRights.openaire
open access
dcterms.isPartOf.issn
1465-9921