dc.contributor.author
Radbruch, Helena
dc.contributor.author
Bremer, Daniel
dc.contributor.author
Mothes, Ronja
dc.contributor.author
Günther, Robert
dc.contributor.author
Rinnenthal, Jan Leo
dc.contributor.author
Pohlan, Julian
dc.contributor.author
Ulbricht, Carolin
dc.contributor.author
Hauser, Anja E.
dc.contributor.author
Niesner, Raluca
dc.date.accessioned
2018-06-08T03:00:04Z
dc.date.available
2015-07-16T12:32:41.077Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/14293
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-18488
dc.description.abstract
The development of intravital Förster Resonance Energy Transfer (FRET) is
required to probe cellular and tissue function in the natural context: the
living organism. Only in this way can biomedicine truly comprehend
pathogenesis and develop effective therapeutic strategies. Here we demonstrate
and discuss the advantages and pitfalls of two strategies to quantify FRET in
vivo—ratiometrically and time-resolved by fluorescence lifetime imaging—and
show their concrete application in the context of neuroinflammation in adult
mice.
en
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
intravital FRET
dc.subject
multi-photon microscopy
dc.subject
fluorescence lifetime imaging
dc.subject
genetically encoded calcium indicators
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
International Journal of Molecular Sciences. - 16 (2015), 5, S. 11713-11727
dc.title.subtitle
Probing Cellular and Tissue Function in Vivo
dcterms.bibliographicCitation.doi
10.3390/ijms160511713
dcterms.bibliographicCitation.url
http://www.mdpi.com/1422-0067/16/5/11713
refubium.affiliation
Charité - Universitätsmedizin Berlin
de
refubium.mycore.fudocsId
FUDOCS_document_000000022840
refubium.note.author
Der Artikel wurde in einer Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000005217
dcterms.accessRights.openaire
open access