Subtitle:
Probing Cellular and Tissue Function in Vivo
Author(s):
Radbruch, Helena; Bremer, Daniel; Mothes, Ronja; Günther, Robert; Rinnenthal, Jan Leo; Pohlan, Julian; Ulbricht, Carolin; Hauser, Anja E.; Niesner, Raluca
Year of publication:
2015
Available Date:
2015-07-16T12:32:41.077Z
Abstract:
The development of intravital Förster Resonance Energy Transfer (FRET) is
required to probe cellular and tissue function in the natural context: the
living organism. Only in this way can biomedicine truly comprehend
pathogenesis and develop effective therapeutic strategies. Here we demonstrate
and discuss the advantages and pitfalls of two strategies to quantify FRET in
vivo—ratiometrically and time-resolved by fluorescence lifetime imaging—and
show their concrete application in the context of neuroinflammation in adult
mice.
Keywords:
intravital FRET
multi-photon microscopy
fluorescence lifetime imaging
genetically encoded calcium indicators
DDC-Classification:
610 Medizin und Gesundheit
Publication Type:
Wissenschaftlicher Artikel
Also published in:
International Journal of Molecular Sciences. - 16 (2015), 5, S. 11713-11727
URL of the Original Publication:
DOI of the Original Publication:
Department/institution:
Charité - Universitätsmedizin Berlin
Comments:
Der Artikel wurde in einer Open-Access-Zeitschrift publiziert.
