dc.contributor.author
Volz, Pierre
dc.contributor.author
Boreham, Alexander
dc.contributor.author
Wolf, Alexander
dc.contributor.author
Kim, Tai-Yang
dc.contributor.author
Balke, Jens
dc.contributor.author
Frombach, Janna
dc.contributor.author
Hadam, Sabrina
dc.contributor.author
Afraz, Zahra
dc.contributor.author
Rancan, Fiorenza
dc.contributor.author
Blume-Peytavi, Ulrike
dc.contributor.author
Vogt, Annika
dc.contributor.author
Alexiev, Ulrike
dc.date.accessioned
2018-06-08T02:55:26Z
dc.date.available
2015-06-15T07:49:21.068Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/14146
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-18343
dc.description.abstract
We report here on the application of laser-based single molecule total
internal reflection fluorescence microscopy (TIRFM) to study the penetration
of molecules through the skin. Penetration of topically applied drug molecules
is often observed to be limited by the size of the respective drug. However,
the molecular mechanisms which govern the penetration of molecules through the
outermost layer of the skin are still largely unknown. As a model compound we
have chosen a larger amphiphilic molecule (fluorescent dye ATTO-Oxa12) with a
molecular weight >700 Da that was applied to excised human skin. ATTO-Oxa12
penetrated through the stratum corneum (SC) into the viable epidermis as
revealed by TIRFM of cryosections. Single particle tracking of ATTO-Oxa12
within SC sheets obtained by tape stripping allowed us to gain information on
the localization as well as the lateral diffusion dynamics of these molecules.
ATTO-Oxa12 appeared to be highly confined in the SC lipid region between
(intercellular space) or close to the envelope of the corneocytes. Three main
distinct confinement sizes of 52 ± 6, 118 ± 4, and 205 ± 5 nm were determined.
We conclude that for this amphiphilic model compound several pathways through
the skin exist.
de
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
single molecule fluorescence microscopy
dc.subject
total internal reflection fluorescence microscopy
dc.subject
single particle tracking
dc.subject
skin penetration
dc.subject
penetration pathways
dc.subject
stratum corneum
dc.subject
tape stripping
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit
dc.title
Application of Single Molecule Fluorescence Microscopy to Characterize the
Penetration of a Large Amphiphilic Molecule in the Stratum Corneum of Human
Skin
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
International Journal of Molecular Sciences. - 16 (2015), 4, S. 6960-6977
dcterms.bibliographicCitation.doi
10.3390/ijms16046960
dcterms.bibliographicCitation.url
http://www.mdpi.com/1422-0067/16/4/6960
refubium.affiliation
Physik
de
refubium.mycore.fudocsId
FUDOCS_document_000000022646
refubium.note.author
Der Artikel wurde in einer Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000005055
dcterms.accessRights.openaire
open access