dc.contributor.author
Jabbar, Abdul
dc.contributor.author
Littlewood, D. Timothy J.
dc.contributor.author
Mohandas, Namitha
dc.contributor.author
Briscoe, Andrew G.
dc.contributor.author
Foster, Peter G.
dc.contributor.author
Müller, Fritz
dc.contributor.author
Samson-Himmelstjerna, Georg von
dc.contributor.author
Jex, Aaron R.
dc.contributor.author
Gasser, Robin B.
dc.date.accessioned
2018-06-08T02:52:27Z
dc.date.available
2015-06-09T07:35:42.966Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/14046
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-18243
dc.description.abstract
Parascaris univalens is an ascaridoid nematode of equids. Little is known
about its epidemiology and population genetics in domestic and wild horse
populations. PCR-based methods are suited to support studies in these areas,
provided that reliable genetic markers are used. Recent studies have shown
that mitochondrial (mt) genomic markers are applicable in such methods, but no
such markers have been defined for P. univalens. Mt genome regions were
amplified from total genomic DNA isolated from P. univalens eggs by long-PCR
and sequenced using Illumina technology. The mt genome was assembled and
annotated using an established bioinformatic pipeline. Amino acid sequences
inferred from all protein-encoding genes of the mt genomes were compared with
those from other ascaridoid nematodes, and concatenated sequences were
subjected to phylogenetic analysis by Bayesian inference. The circular mt
genome was 13,920 bp in length and contained two ribosomal RNA, 12 protein-
coding and 22 transfer RNA genes, consistent with those of other ascaridoids.
Phylogenetic analysis of the concatenated amino acid sequence data for the 12
mt proteins showed that P. univalens was most closely related to Ascaris
lumbricoides and A. suum, to the exclusion of other ascaridoids. This mt
genome representing P. univalens now provides a rich source of genetic markers
for future studies of the genetics and epidemiology of this parasite and its
congener, P. equorum. This focus is significant, given that there is no
published information on the specific prevalence and distribution of P.
univalens infection in domestic and wild horse populations.
en
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Parascaris univalens (Nematoda: Ascaridida)
dc.subject
Mitochondrial genome
dc.subject
Genetic markers
dc.subject
Population genetics
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft
dc.title
The mitochondrial genome of Parascaris univalens - implications for a
“forgotten” parasite
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Parasites & Vectors. - 7 (2014), 1, S.428
dc.identifier.sepid
44379
dcterms.bibliographicCitation.doi
10.1186/1756-3305-7-428
dcterms.bibliographicCitation.url
http://dx.doi.org/10.1186/1756-3305-7-428
refubium.affiliation
Veterinärmedizin
de
refubium.affiliation.other
Institut für Parasitologie und Tropenveterinärmedizin
refubium.mycore.fudocsId
FUDOCS_document_000000022569
refubium.note.author
Der Artikel wurde in einer Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000005013
dcterms.accessRights.openaire
open access
dcterms.isPartOf.issn
1756-3305