The paper presents a database of hydrochemical and hydraulic groundwater measurements of a younger Pleistocene multilayered, unconfined/confined aquifer system in NE Germany. The Institute of Landscape Hydrology of the Leibniz Centre for Agricultural Landscape Research (ZALF) operates seven groundwater monitoring wells in the Quillow catchment located in the Uckermark region (federal state of Brandenburg, Germany). From July 2000 to March 2014, water samples were collected periodically on different days of the year and at depths between 3 and 5 m (shallow wells) and 16 and 24 m (deeper wells) below the surface. The parameters pH value, redox potential, electric conductivity, water temperature, oxygen content, spectral absorption coefficient and concentration of hydrogen carbonate, ammonium, phosphate, chloride, bromide, nitrite, sulfate, sodium, potassium, magnesium, calcite, dissolved organic carbon, iron(II) and manganese were determined for each sample (doi:10.4228/ZALF.2000.266). The measurements, taken over a period of 14 years, include a high variation of hydraulic situations represented by a corresponding database of 19 000 recorded groundwater heads. The hydraulic head was measured between 2000 and 2014