id,collection,dc.contributor.author,dc.date.accessioned,dc.date.available,dc.date.issued,dc.description.abstract[en],dc.identifier.uri,dc.language,dc.subject.ddc,dc.subject[en],dc.title,dc.type,dcterms.accessRights.openaire,dcterms.bibliographicCitation.doi,dcterms.bibliographicCitation.journaltitle,dcterms.bibliographicCitation.number,dcterms.bibliographicCitation.originalpublishername,dcterms.bibliographicCitation.pageend,dcterms.bibliographicCitation.pagestart,dcterms.bibliographicCitation.pmid,dcterms.bibliographicCitation.volume,dcterms.isPartOf.eissn,dcterms.isPartOf.issn,dcterms.rightsHolder.note,dcterms.rightsHolder.url,refubium.affiliation,refubium.note.author[de],refubium.note.author[en],refubium.resourceType.isindependentpub "3af04ece-03b2-49bf-92ad-6b052fdab9d1","fub188/15","Hou, Xiaoxiao||Hossini, Amir M.||Nikolakis, Georgios||Balthasar, Ottfried||Kurtz, Andreas||Zouboulis, Christos C.","2022-08-16T12:40:54Z","2022-08-16T12:40:54Z","2021","Background: Hidradenitis suppurativa/acne inversa (HS) is a chronic, recurrent inflammatory skin disease. Its pivotal pathogenetic event is believed to be the occlusion of the hair follicle generating a perifollicular lympho-histiocytic inflammation. However, knowledge of the exact HS pathogenesis requires further research. Objective: To develop a human HS model applicable in preclinical research which could help to understand the pathophysiology of HS and to determine the action of therapeutic candidates. Methods: The 3D-SeboSkin technology was applied to maintain explants of involved and uninvolved skin of HS patients ex vivo for 3 days. Detection of differential expression of previously detected HS biomarkers was performed by immunohistochemistry in a group of female patients (n = 9, mean age 37.2 +/- 8.4 years). Results: The application of the 3D-SeboSkin model preserved the structural integrity of lesional and perilesional HS skin ex vivo, as previously described for healthy skin. Moreover, the HS 3D-SeboSkin setting maintained the differential expression and pattern of several HS biomarkers (S100A9, KRT16, SERPINB3) in epidermal and dermal tissue and the appendages. Conclusion: We have validated HS 3D-SeboSkin as a reproducible, human model, which is appropriate for preclinical lesional and perilesional HS skin studies ex vivo.","https://refubium.fu-berlin.de/handle/fub188/35907||http://dx.doi.org/10.17169/refubium-35622","eng","600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit","Hidradenitis suppurativa||Skin model||Ex vivo||3D-SeboSkin","3D-SeboSkin Model for Human ex vivo Studies of Hidradenitis Suppurativa/Acne Inversa","Wissenschaftlicher Artikel","open access","10.1159/000515955","Dermatology","2","Karger","243","236","34077928","238","1421-9832","1018-8665","Copyright applies in this work.","http://rightsstatements.org/vocab/InC/1.0/","Charité - Universitätsmedizin Berlin","Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG geförderten) Allianz- bzw. Nationallizenz frei zugänglich.","This publication is shared with permission of the rights owner and made freely accessible through a DFG (German Research Foundation) funded license at either an alliance or national level.","no"