id,collection,dc.contributor.author,dc.date.accessioned,dc.date.available,dc.date.issued,dc.description.abstract[en],dc.identifier.uri,dc.language,dc.rights.uri,dc.subject.ddc,dc.subject[en],dc.title,dc.type,dcterms.accessRights.openaire,dcterms.bibliographicCitation.doi,dcterms.bibliographicCitation.journaltitle,dcterms.bibliographicCitation.number,dcterms.bibliographicCitation.originalpublishername,dcterms.bibliographicCitation.pageend,dcterms.bibliographicCitation.pagestart,dcterms.bibliographicCitation.pmid,dcterms.bibliographicCitation.volume,dcterms.isPartOf.eissn,dcterms.isPartOf.issn,refubium.affiliation,refubium.funding,refubium.resourceType.isindependentpub "ad78c6f9-d358-4cd8-8e13-42caddc71a29","fub188/15","Scholz, Josephine||Kuhrau, Julia||Heinrich, Frederik||Heinz, Gitta Anne||Hutloff, Andreas||Worm, Margitta||Heine, Guido","2022-02-08T14:38:09Z","2022-02-08T14:38:09Z","2020","Background Vitamin A regulates the adaptive immune response and a modulatory impact on type I allergy is discussed. The cellular mechanisms are largely unknown. Objective To determine the vitamin A-responding specific lymphocyte reaction in vivo. Methods Antigen-specific B and T lymphocytes were analyzed in an adoptive transfer airway inflammation mouse model in response to 9-cis retinoic acid (9cRA) and after lymphocyte-specific genetic targeting of the receptor RAR alpha. Flow cytometry, quantitative PCR, next-generation sequencing, and specific Ig-ELISA were used to characterize the cells functionally. Results Systemic 9cRA profoundly enhanced the specific IgA-secreting B-cell frequencies in the lung tissue and serum IgA while reducing serum IgE concentrations. RAR alpha overexpression in antigen-specific B cells promoted differentiation into plasmablasts at the expense of germinal center B cells. In antigen-specific T cells, RAR alpha strongly promoted the differentiation of T follicular helper cells followed by an enhanced germinal center response. Conclusions 9cRA signaling via RAR alpha impacts the allergen-specific immunoglobulin response directly by the differentiation of B cells and indirectly by promoting T follicular helper cells.","https://refubium.fu-berlin.de/handle/fub188/33942||http://dx.doi.org/10.17169/refubium-33661","eng","https://creativecommons.org/licenses/by-nc-nd/4.0/","600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit","9-cis retinoic acid||allergy||RAR||Tfh cells||vitamin A","Vitamin A controls the allergic response through T follicular helper cell as well as plasmablast differentiation","Wissenschaftlicher Artikel","open access","10.1111/all.14581","Allergy","4","Wiley","1122","1109","32895937","76","1398-9995","0105-4538","Charité - Universitätsmedizin Berlin","DEAL Wiley","no"