id,collection,dc.contributor.author,dc.date.accessioned,dc.date.available,dc.date.issued,dc.description.abstract[en],dc.format.extent,dc.identifier.uri,dc.language,dc.rights.uri,dc.subject.ddc,dc.subject[en],dc.title,dc.type,dcterms.accessRights.openaire,dcterms.bibliographicCitation.doi,dcterms.bibliographicCitation.journaltitle,dcterms.bibliographicCitation.number,dcterms.bibliographicCitation.pageend,dcterms.bibliographicCitation.pagestart,dcterms.bibliographicCitation.url,dcterms.bibliographicCitation.volume,dcterms.isPartOf.eissn,refubium.affiliation,refubium.affiliation.other,refubium.funding[],refubium.note.author[],refubium.resourceType.isindependentpub,refubium.resourceType.provider "e077bbc6-fc97-4845-a6a3-5e0875502dd9","fub188/16","Nerlich, Andreas||Janze, Nina||Goethe, Ralph","2022-01-03T11:36:54Z","2022-01-03T11:36:54Z","2021","Interleukin-36α is a novel member of the IL-1 cytokine family that is highly expressed in epithelial tissues and several myeloid-derived cell types after induction. The transcription factor (TF) C/EBPβ binds specifically to an essential half-CRE•C/EBP motif in the Il36a promoter to induce Il36a expression upon LPS stimulation. C/EBPs regulate gene expression by binding to recognition sequences that can contain 5′-cytosine-phosphate-guanine-3′ dinucleotides (CpG), whose methylation can influence TF binding and gene expression. Herein we show that the half-CRE•C/EBP element in the Il36a promoter is differentially methylated in the murine RAW264.7 macrophage cell line and in primary murine macrophages. We demonstrate that C/EBPβ binding to the half-CRE•C/EBP element in the Il36a promoter following LPS stimulation is insensitive to CpG methylation and that methylation of the CpG in the half-CRE•C/EBP element does not alter LPS-induced Il36a promoter activity which correlated with similar Il36a mRNA copy numbers and pro-IL-36α protein amount in both cell types. Taken together, our data indicate that C/EBPβ binding to the half-CRE•C/EBP element and subsequent gene activation occurs independently of the CpG methylation status of the half-CRE•C/EBP motif and underlines the potential of C/EBPs to recognize methylated as well as unmethylated motifs.","9 Seiten","https://refubium.fu-berlin.de/handle/fub188/32906||http://dx.doi.org/10.17169/refubium-32632","eng","https://creativecommons.org/licenses/by/4.0/","500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie","Gene regulation||Interleukins||cytokine","Analysis of Il36a induction by C/EBPβ via a half-CRE•C/EBP element in murine macrophages in dependence of its CpG methylation level","Wissenschaftlicher Artikel","open access","10.1038/s41435-021-00153-5","Genes & Immunity","7-8","321","313","https://doi.org/10.1038/s41435-021-00153-5","22","1476-5470","Veterinärmedizin","Tiermedizinisches Zentrum für Resistenzforschung (TZR)","Springer Nature DEAL","Die Publikation wurde aus Open Access Publikationsgeldern der Freien Universität Berlin gefördert.","no","WoS-Alert"