id,collection,dc.contributor.author,dc.date.accessioned,dc.date.available,dc.date.issued,dc.description.abstract[en],dc.identifier.uri,dc.language,dc.rights.uri,dc.subject.ddc,dc.subject[en],dc.title,dc.type,dcterms.accessRights.openaire,dcterms.bibliographicCitation.articlenumber,dcterms.bibliographicCitation.doi,dcterms.bibliographicCitation.journaltitle,dcterms.bibliographicCitation.number,dcterms.bibliographicCitation.originalpublishername,dcterms.bibliographicCitation.pmid,dcterms.bibliographicCitation.volume,dcterms.isPartOf.issn,refubium.affiliation,refubium.resourceType.isindependentpub "b689529c-c4de-4062-b1ea-d287b06a3039","fub188/15","Pappritz, Kathleen||Savvatis, Konstantinos||Koschel, Annika||Miteva, Kapka||Tschöpe, Carsten||Linthout, Sophie van","2019-04-16T11:26:57Z","2019-04-16T11:26:57Z","2018","Cardiac fibroblasts play an important role in the regulation of the extracellular matrix and are newly recognized as inflammatory supporter cells. Interferon (IFN)-gamma is known to counteract transforming growth factor (TGF)-beta 1-induced myofibroblast differentiation. This study aims at investigating in vitro how IFN-gamma affects TGF-beta 1-induced monocyte attraction. Therefore, C4 fibroblasts and fibroblasts obtained by outgrowth culture from the left ventricle (LV) of male C57BL6/j mice were stimulated with TGF-beta 1, IFN-gamma and TGF-beta 1 + IFN-gamma. Confirming previous studies, IFN-gamma decreased the TGF-beta 1-induced myofibroblast differentiation, as obviated by lower collagen I, III, alpha-smooth muscle actin (alpha-SMA), lysyl oxidase (Lox)-1 and lysyl oxidase-like (LoxL)-2 levels in TGF-beta 1 + IFN-gamma-versus TGF-beta 1-stimulated cardiac fibroblasts. TGF-beta 1 + IFN-gamma-stimulated C4 and cardiac fibroblasts displayed a higher CC-chemokine ligand (CCL) 2, CCL7 and chemokine C-X3-C motif ligand (Cx3CL1) release versus sole TGF-beta 1- stimulated fibroblasts. Analysis of migrated monocyte subsets towards the different conditioned media further revealed that sole TGF-beta 1- and IFN-gamma-conditioned media particularly attracted Ly6C(low) and Ly6C(high) monocytes, respectively, as compared to control media. In line with theses findings, TGF-beta 1 + IFN-gamma-conditioned media led to a lower Ly6C(low)/Ly6C(high) monocyte migration ratio compared to sole TGF-beta 1 treatment. These differences in monocyte migration reflect the complex interplay of pro-inflammatory cytokines and pro-fibrotic factors in cardiac remodelling and inflammation.","https://refubium.fu-berlin.de/handle/fub188/24437||http://dx.doi.org/10.17169/refubium-2209","eng","https://creativecommons.org/licenses/by/4.0/","600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit","Cardiac fibroblasts||extracellular matrix","Cardiac (myo)fibroblasts modulate the migration of monocyte subsets","Wissenschaftlicher Artikel","open access","5575","10.1038/s41598-018-23881-7","Scientific Reports","1","Nature Publishing Group","29615815","8","2045-2322","Charité - Universitätsmedizin Berlin","no"