id,collection,dc.contributor.author,dc.date.accessioned,dc.date.available,dc.date.issued,dc.description.abstract[en],dc.format.extent,dc.identifier.uri,dc.language,dc.rights.uri,dc.subject.ddc,dc.subject[en],dc.title,dc.type,dcterms.accessRights.openaire,dcterms.bibliographicCitation.doi,dcterms.bibliographicCitation.journaltitle,dcterms.bibliographicCitation.pageend,dcterms.bibliographicCitation.pagestart,dcterms.bibliographicCitation.url,dcterms.bibliographicCitation.volume,dcterms.isPartOf.issn,dcterms.rightsHolder.note,dcterms.rightsHolder.url,refubium.affiliation,refubium.affiliation.other,refubium.resourceType.isindependentpub "62fe7528-7379-441f-aae2-6218f07c6ddf","fub188/16","Bommer, Martin||Coates, Leighton||Dau, Holger||Zouni, Athina||Dobbek, Holger","2018-12-14T10:21:10Z","2018-12-14T10:21:10Z","2017","The PsbO protein of photosystem II stabilizes the active-site manganese cluster and is thought to act as a proton antenna. To enable neutron diffraction studies, crystals of the β-barrel core of PsbO were grown in capillaries. The crystals were optimized by screening additives in a counter-diffusion setup in which the protein and reservoir solutions were separated by a 1% agarose plug. Crystals were cross-linked with glutaraldehyde. Initial neutron diffraction data were collected from a 0.25 mm3 crystal at room temperature using the MaNDi single-crystal diffractometer at the Spallation Neutron Source, Oak Ridge National Laboratory.","7 S.","https://refubium.fu-berlin.de/handle/fub188/23583||http://dx.doi.org/10.17169/refubium-1369","eng","http://www.fu-berlin.de/sites/refubium/rechtliches/Nutzungsbedingungen","500 Naturwissenschaften und Mathematik::530 Physik::530 Physik","photosystem II||PsbO||counter-diffusion||agarose||neutron diffraction||cross-linking","Protein crystallization and initial neutron diffraction studies of the photosystem II subunit PsbO","Wissenschaftlicher Artikel","open access","10.1107/S2053230X17012171","Acta Crystallographica Section F: Structural Biology Communications","531","525","https://doi.org/10.1107/S2053230X17012171","F73","2053-230X","Copyright des Verlages","http://journals.iucr.org/services/copyrightpolicy.html","Physik","Institut für Experimentalphysik:::0e15dd66-95f1-40d5-8307-e68203f86a76:::600","no"