id,collection,dc.contributor.author[],dc.date.accessioned[],dc.date.available[],dc.date.issued[],dc.description.abstract[en],dc.format.extent[],dc.identifier.uri,dc.identifier.uri[],dc.language[],dc.rights.uri[],dc.subject.ddc,dc.subject[],dc.title[],dc.type[],dcterms.accessRights.openaire,dcterms.bibliographicCitation.doi[],dcterms.bibliographicCitation.url[],dcterms.bibliographicCitation[],refubium.affiliation[de],refubium.funding.id[],refubium.funding[],refubium.mycore.derivateId[],refubium.mycore.fudocsId[],refubium.note.author[],refubium.resourceType.isindependentpub[] "35d801df-de91-4bc2-8d92-b2b071292c50","fub188/16","Ebner, Friederike||Schwiertz, Patrycja||Steinfelder, Svenja||Pieper, Robert||Zentek, Jürgen||Schuetze, Nicole||Baums, Christoph||Alber, Gottfried||Geldhof, Peter||Hartmann, Susanne","2018-06-08T11:09:41Z","2017-06-08T06:04:54.463Z","2017","There is growing interest in studying host–pathogen interactions in human- relevant large animal models such as the pig. Despite the progress in developing immunological reagents for porcine T cell research, there is an urgent need to directly assess pathogen-specific T cells—an extremely rare population of cells, but of upmost importance in orchestrating the host immune response to a given pathogen. Here, we established that the activation marker CD154 (CD40L), known from human and mouse studies, identifies also porcine antigen-reactive CD4+ T lymphocytes. CD154 expression was upregulated early after antigen encounter and CD4+CD154+ antigen-reactive T cells coexpressed cytokines. Antigen-induced expansion and autologous restimulation enabled a time- and dose-resolved analysis of CD154 regulation and a significantly increased resolution in phenotypic profiling of antigen-responsive cells. CD154 expression identified T cells responding to staphylococcal Enterotoxin B superantigen stimulation as well as T cells responding to the fungus Candida albicans and T cells specific for a highly prevalent intestinal parasite, the nematode Ascaris suum during acute and trickle infection. Antigen-reactive T cells were further detected after immunization of pigs with a single recombinant bacterial antigen of Streptococcus suis only. Thus, our study offers new ways to study antigen-specific T lymphocytes in the pig and their contribution to host–pathogen interactions.","15 S.","http://dx.doi.org/10.17169/refubium-25015","https://refubium.fu-berlin.de/handle/fub188/21727","eng","http://creativecommons.org/licenses/by/4.0/","600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft","antigen-specific||pig||porcine CD4 T cell||CD154||CD40 ligand||Ascaris suum||Candida albicans||Streptococcus suis","Pathogen-Reactive T Helper Cell Analysis in the Pig","Wissenschaftlicher Artikel","open access","10.3389/fimmu.2017.00565","http://doi.org/10.3389/fimmu.2017.00565","Front. Immunol. - 8 (2017), Artikel Nr. 565","Veterinärmedizin","Inst. Mitgliedschaft bei Frontiers","Sonstige","FUDOCS_derivate_000000008286","FUDOCS_document_000000027134","Der Artikel wurde in einer reinen Open-Access-Zeitschrift publiziert.","no"