id,collection,dc.contributor.author,dc.date.accessioned,dc.date.available,dc.date.issued,dc.description.abstract[en],dc.format.extent,dc.identifier.uri,dc.language,dc.rights.uri,dc.subject.ddc,dc.title,dc.type,dcterms.accessRights.openaire,dcterms.bibliographicCitation,dcterms.bibliographicCitation.doi,dcterms.bibliographicCitation.url,refubium.affiliation[de],refubium.mycore.derivateId,refubium.mycore.fudocsId,refubium.note.author,refubium.resourceType.isindependentpub "643ec772-c0e8-4a43-b5aa-66975cd3ad1d","fub188/15","Kociok, Norbert||Crespo-Garcia, Sergio||Liang, Yong||Klein, Sabrina V.||Nürnberg, Christina||Reichhart, Nadine||Skosyrski, Sergej||Moritz, Eva||Maier, Anna-Karina||Brunken, William J.||Strauß, Olaf||Koch, Manuel||Joussen, Antonia M.","2018-06-08T03:04:37Z","2016-02-22T12:19:30.910Z","2016","Netrins are a family of matrix-binding proteins that function as guidance signals. Netrin-4 displays pathologic roles in tumorigenesis and neovascularization. To answer the question whether netrin-4 acts either pro- or anti-angiogenic, angiogenesis in the retina was assessed in Ntn-4−/− mice with oxygen-induced retinopathy (OIR) and laser-induced choroidal neovascularization (CNV), mimicking hypoxia-mediated neovascularization and inflammatory mediated angiogenesis. The basement membrane protein netrin-4 was found to be localised to mature retinal blood vessels. Netrin-4, but not netrin-1 mRNA expression, increased in response to relative hypoxia and recovered to normal levels at the end of blood vessel formation. No changes in the retina were found in normoxic Ntn-4−/− mice. In OIR, Ntn-4−/− mice initially displayed larger avascular areas which recovered faster to revascularization. Ganzfeld electroretinography showed faster recovery of retinal function in Ntn-4−/− mice. Expression of netrin receptors, Unc5H2 (Unc-5 homolog B, C. elegans) and DCC (deleted in colorectal carcinoma), was found in Müller cells and astrocytes. Laser-induced neovascularization in Nnt-4−/− mice did not differ to that in the controls. Our results indicate a role for netrin-4 as an angiogenesis modulating factor in O2-dependent vascular homeostasis while being less important during normal retinal developmental angiogenesis or during inflammatory neovascularization.","13 S.","https://refubium.fu-berlin.de/handle/fub188/14463||http://dx.doi.org/10.17169/refubium-18655","eng","http://creativecommons.org/licenses/by/4.0/","600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit","Lack of netrin-4 modulates pathologic neovascularization in the eye","Wissenschaftlicher Artikel","open access","Scientific Reports. - 6 (2016), Artikel Nr. 18828","10.1038/srep18828","http://www.nature.com/articles/srep18828","Charité - Universitätsmedizin Berlin","FUDOCS_derivate_000000006017","FUDOCS_document_000000023937","Der Artikel wurde in einer reinen Open-Access-Zeitschrift publiziert.","no"