This AcunaHidalgo_Armitage_readme.txt file was generated on 2022-02-08 by SOPHIE ARMITAGE & BEATRIZ ACUÑA HIDALGO GENERAL INFORMATION 1. Title of Datasets: AcunaHidalgo_Armitage_dataset 2. Author Information Principal Investigator Contact Information Name: Sophie Armitage Institution: Freie Universität Berlin Address: Königin-Luise-Str. 1-3, 14195 Berlin, Germany Email: sophie.armitage@fu-berlin.de 3. Date of data collection (single date, range, approximate date): 2019-07-15 to 2019-12-19 4. Geographic location of data collection: Berlin, Germany 5. Information about funding sources that supported the collection of the data: This work was funded by the DFG grant numbers AR 872/3-1, AR 872/4-1 and AR 872/7-1 SHARING/ACCESS INFORMATION 1. Licenses/restrictions placed on the data: No 2. Links to publications that cite or use the data: Acuña Hidalgo & Armitage (2022) Host resistance to bacterial infection varies over time, but is not affected by a previous exposure to the same pathogen. Frontiers in Physiology. DOI not available at the time of submission of these data. 3. Was data derived from another source? no 4. Recommended citation for this dataset: http://dx.doi.org/10.17169/refubium-33781 DATA & FILE OVERVIEW 1. File List: model1a.csv fly survival seven days after primary exposure to Lactococcus lactis model1b.csv fly survival seven days after primary exposure to Providencia burhodogranariea model2a.csv fly survival (censor data) for seven days after secondary exposure to Lactococcus lactis model2b.csv fly survival (censor data) for seven days after secondary exposure to Providencia burhodogranariea model3a.csv bacterial load one day after secondary exposure with Lactococcus lactis of flies carrying a low load (<18268.63 CFUs/fly) model3b.csv bacterial load one day after secondary exposure with Lactococcus lactis of flies carrying a high load (>18268.63 CFUs/fly) model3c.csv bacterial load one day after secondary exposure with Providencia burhodogranariea of flies carrying a low load (<14383.78 CFUs/fly) model3d.csv bacterial load one day after secondary exposure with Providencia burhodogranariea of flies carrying a high load (>14383.78 CFUs/fly) model3e.csv bacterial load one day after secondary exposure with Providencia burhodogranariea of flies carrying a low load (<14383.78 CFUs/fly) excluding three data points around or above 0.5 Cook's distance model4a.csv proportion of flies with low and high loads one day after secondary exposure with Lactococcus lactis model4b.csv proportion of flies with low and high loads one day after secondary exposure with Providencia burhodogranariea model5a.csv bacterial load seven days after secondary exposure with Lactococcus lactis model5b.csv bacterial load seven days after secondary exposure with Providencia burhodogranariea 2. Relationship between files, if important: Data was collected in the same experimental replicates (numbered from 1 to 4) for all data files except for: - model2a.csv and model2b.csv, which contain data from replicates 3, 4 and two additional experimental replicates numbered 5 and 6 - model1a.csv and model1b, which contain data from replicates 1-5 3. Are there multiple versions of the dataset? no METHODOLOGICAL INFORMATION 1. Description of methods used for collection/generation of data: The experiment was carried out and data collected in the methods described in Acuña Hidalgo & Armitage (in press) Host resistance to bacterial infection varies over time, but is not affected by a previous exposure to the same pathogen. Frontiers in Physiology. 2. Methods for processing the data: The data files contain the raw data. 3. Instrument- or software-specific information needed to interpret the data: No specific software is needed to interpret the data. 4. Describe any quality-assurance procedures performed on the data: At data entry, the data was double-checked for accuracy. DATA-SPECIFIC INFORMATION FOR: model1a.csv 1. Number of variables: 4 2. Number of cases/rows: 124 3. Variable List: Each row contains data from 10 flies that were kept together in one vial after the previous exposure (priming) with Lactococcus lactis. rep the experiments were repeated 5 times. The experiments are numbered 1 through 5. priming the previous exposure that the flies received. They were injected with r = ringer’s, llhk = heat-killed L. lactis, llform = formaldehyde inactivated L. lactis, llcomb = a combination of heat-killed and formaldehyde inactivated L. lactis. survived number of flies that were alive 7 days after the previous exposure died number of flies that were dead 7 days after the previous exposure 4. Missing data codes: There is no missing data DATA-SPECIFIC INFORMATION FOR: model1b.csv 1. Number of variables: 4 2. Number of cases/rows: 124 3. Variable List: Each row contains data from 10 flies that were kept together in one vial after the previous exposure (priming) with Providencia burhodogranariea. rep the experiments were repeated 5 times. The experiments are numbered 1 through 5. priming the previous exposure that the flies received. They were injected with r = ringer’s, pbhk = heat-killed P. burhodogranariea, pbform = formaldehyde inactivated P. burhodogranariea, pbcomb = a combination of heat-killed and formaldehyde inactivated P. burhodogranariea. survived number of flies that were alive 7 days after the previous exposure died number of flies that were dead 7 days after the previous exposure 4. Missing data codes: There is no missing data DATA-SPECIFIC INFORMATION FOR: model2a.csv 1. Number of variables: 5 2. Number of cases/rows: 378 3. Variable List: Each row contains data from individual flies kept in a vial containing six flies for seven days after the secondary exposure (challenge) with Lactococcus lactis. Vials were flipped every 3-4 days. rep the experiments were repeated 4 times. The experiments are numbered 3 through 6. priming the previous exposure that the flies received. They were injected with r = ringer’s, llhk = heat-killed L. lactis, llform = formaldehyde inactivated L. lactis, llcomb = a combination of heat-killed and formaldehyde inactivated L. lactis. vial_id Identification number of the vial where the individual fly was contained in groups of six flies/vial day day post-challenge in which the survival status of the fly was assayed censor survival status of the fly: 1 = dead ; 0 = alive 4. Missing data codes: There is no missing data DATA-SPECIFIC INFORMATION FOR: model2b.csv 1. Number of variables: 5 2. Number of cases/rows: 383 3. Variable List: Each row contains data from individual flies kept in a vial containing six flies for seven days after the secondary exposure (challenge) with Providencia burhodogranariea. Vials were flipped every 3-4 days. rep the experiments were repeated 4 times. The experiments are numbered 3 through 6. priming the previous exposure that the flies received. They were injected with r = ringer’s, pbhk = heat-killed P. burhodogranariea, pbform = formaldehyde inactivated P. burhodogranariea, pbcomb = a combination of heat-killed and formaldehyde inactivated P. burhodogranariea. vial_id Identification number of the vial where the individual fly was contained in groups of six flies/vial day day post-challenge in which the survival status of the fly was assayed censor survival status of the fly: 1 = dead ; 0 = alive 4. Missing data codes: There is no missing data DATA-SPECIFIC INFORMATION FOR: model3a.csv 1. Number of variables: 3 2. Number of cases/rows: 72 3. Variable List: Each row contains data from individual flies kept in a vial containing six flies for one day after the secondary exposure (challenge) with Lactococcus lactis. Only data from flies that presented a low load i.e. lower than the cut-off point of 18268.63 colony-forming units/fly. rep the experiments were repeated 4 times. The experiments are numbered 1 through 4. priming the previous exposure that the flies received. They were injected with r = ringer’s, llhk = heat-killed L. lactis, llform = formaldehyde inactivated L. lactis, llcomb = a combination of heat-killed and formaldehyde inactivated L. lactis. bl number of bacterial colony-forming units (bacterial load) of the individual fly assayed at one day post-secondary exposure 4. Missing data codes: There is no missing data DATA-SPECIFIC INFORMATION FOR: model3b.csv 1. Number of variables: 3 2. Number of cases/rows: 32 3. Variable List: Each row contains data from individual flies kept in a vial containing six flies for one day after the secondary exposure (challenge) with Lactococcus lactis. Only data from flies that presented a high load i.e. higher than the cut-off point of 18268.63 colony-forming units/fly. rep the experiments were repeated 4 times. The experiments are numbered 1 through 4. priming the previous exposure that the flies received. They were injected with r = ringer’s, llhk = heat-killed L. lactis, llform = formaldehyde inactivated L. lactis, llcomb = a combination of heat-killed and formaldehyde inactivated L. lactis. bl number of bacterial colony-forming units (bacterial load) of the individual fly assayed at one day post-secondary exposure 4. Missing data codes: There is no missing data DATA-SPECIFIC INFORMATION FOR: model3c.csv 1. Number of variables: 3 2. Number of cases/rows: 67 3. Variable List: Each row contains data from individual flies kept in a vial containing six flies for one day after the secondary exposure (challenge) with Providencia burhodogranariea. Only data from flies that presented a low load i.e. lower than the cut-off point of 14383.78 colony-forming units/fly. rep the experiments were repeated 4 times. The experiments are numbered 1 through 4. priming the previous exposure that the flies received. They were injected with r = ringer’s, pbhk = heat-killed P. burhodogranariea, pbform = formaldehyde inactivated P. burhodogranariea, pbcomb = a combination of heat-killed and formaldehyde inactivated P. burhodogranariea. bl number of bacterial colony-forming units (bacterial load) of the individual fly assayed at one day post-secondary exposure 4. Missing data codes: There is no missing data DATA-SPECIFIC INFORMATION FOR: model3d.csv 1. Number of variables: 3 2. Number of cases/rows: 41 3. Variable List: Each row contains data from individual flies kept in a vial containing six flies for one day after the secondary exposure (challenge) with Providencia burhodogranariea. Only data from flies that presented a high load i.e. higher than the cut-off point of 14383.78 colony-forming units/fly. rep the experiments were repeated 4 times. The experiments are numbered 1 through 4. priming the previous exposure that the flies received. They were injected with r = ringer’s, pbhk = heat-killed P. burhodogranariea, pbform = formaldehyde inactivated P. burhodogranariea, pbcomb = a combination of heat-killed and formaldehyde inactivated P. burhodogranariea. bl number of bacterial colony-forming units (bacterial load) of the individual fly assayed at one day post-secondary exposure 4. Missing data codes: There is no missing data DATA-SPECIFIC INFORMATION FOR: model3e.csv 1. Number of variables: 3 2. Number of cases/rows: 64 3. Variable List: Each row contains data from individual flies kept in a vial containing six flies for one day after the secondary exposure (challenge) with Providencia burhodogranariea. Only data from flies that presented a low load i.e. lower than the cut-off point of 14383.78 colony-forming units/fly. Three flies that were around or above 0.5 Cook's distance were excluded from model3e (See Methods for more information). rep the experiments were repeated 4 times. The experiments are numbered 1 through 4. priming the previous exposure that the flies received. They were injected with r = ringer’s, pbhk = heat-killed P. burhodogranariea, pbform = formaldehyde inactivated P. burhodogranariea, pbcomb = a combination of heat-killed and formaldehyde inactivated P. burhodogranariea. bl number of bacterial colony-forming units (bacterial load) of the individual fly assayed at one day post-secondary exposure 4. Missing data codes: There is no missing data DATA-SPECIFIC INFORMATION FOR: model4a.csv 1. Number of variables: 5 2. Number of cases/rows: 12 3. Variable List: Each row contains data from individual experimental repeats of the bacterial load assay for day one after the secondary exposure (challenge) with Lactococcus lactis. rep the experiments were repeated 4 times. The experiments are numbered 1 through 4. priming the previous exposure that the flies received. They were injected with r = ringer’s, llhk = heat-killed L. lactis, llform = formaldehyde inactivated L. lactis, llcomb = a combination of heat-killed and formaldehyde inactivated L. lactis. low number of flies with a bacterial load lower than the cut-off points of 18268.63 colony-forming units/fly. high number of flies with a bacterial load higher than the cut-off points of 18268.63 colony-forming units/fly. total total number of flies assayed for each repeat (low + high) 4. Missing data codes: There is no missing data DATA-SPECIFIC INFORMATION FOR: model4b.csv 1. Number of variables: 5 2. Number of cases/rows: 12 3. Variable List: Each row contains data from individual experimental repeats of the bacterial load assay for day one after the secondary exposure (challenge) with Providencia burhodogranariea. rep the experiments were repeated 4 times. The experiments are numbered 1 through 4. priming the previous exposure that the flies received. They were injected with r = ringer’s, pbhk = heat-killed P. burhodogranariea, pbform = formaldehyde inactivated P. burhodogranariea, pbcomb = a combination of heat-killed and formaldehyde inactivated P. burhodogranariea. low number of flies with a bacterial load lower than the cut-off points of 14383.78 colony-forming units/fly. high number of flies with a bacterial load higher than the cut-off points of 14383.78 colony-forming units/fly. total total number of flies assayed for each repeat (low + high) 4. Missing data codes: There is no missing data DATA-SPECIFIC INFORMATION FOR: model5a.csv 1. Number of variables: 3 2. Number of cases/rows: 103 3. Variable List: Each row contains data from individual flies kept in a vial containing six flies for seven days after the secondary exposure (challenge) with Lactococcus lactis. Vials were flipped every 3-4 days. rep the experiments were repeated 4 times. The experiments are numbered 1 through 4. priming the previous exposure that the flies received. They were injected with r = ringer’s, llhk = heat-killed L. lactis, llform = formaldehyde inactivated L. lactis, llcomb = a combination of heat-killed and formaldehyde inactivated L. lactis. bl number of bacterial colony-forming units (bacterial load) of the individual fly assayed at seven days post-secondary exposure 4. Missing data codes: There is no missing data DATA-SPECIFIC INFORMATION FOR: model5b.csv 1. Number of variables: 3 2. Number of cases/rows: 100 3. Variable List: Each row contains data from individual flies kept in a vial containing six flies for seven days after the secondary exposure (challenge) with Providencia burhodogranariea. Vials were flipped every 3-4 days. rep the experiments were repeated 4 times. The experiments are numbered 1 through 4. priming the previous exposure that the flies received. They were injected with r = ringer’s, pbhk = heat-killed P. burhodogranariea, pbform = formaldehyde inactivated P. burhodogranariea, pbcomb = a combination of heat-killed and formaldehyde inactivated P. burhodogranariea. bl number of bacterial colony-forming units (bacterial load) of the individual fly assayed at seven days post-secondary exposure 4. Missing data codes: There is no missing data